Sodium Fluoride Inhibits MMP-2 and MMP-9
Sodium Fluoride Inhibits MMP-2 and MMP-9
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DOI：
10.1177/0022034513511820
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发表时间：
2014-01-01
影响因子：
7.6
通讯作者：
Buzalaf, M. A. R.
中科院分区：
文献类型：
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作者：
Kato, M. T.;Bolanho, A.;Buzalaf, M. A. R.
氟化物（F）通过有利地干扰脱矿-再矿化过程来预防龋齿的重要性已得到充分证实，但其抑制基质金属蛋白酶（MMP）的能力（这也可能有助于预防牙本质龋齿）尚未得到研究。本研究评估了 F 抑制唾液和纯化的人明胶酶 MMP-2 和 -9 的能力。唾液采集自 10 名健康个体。将合并的唾液离心，将上清液在 37°C 下孵育 1 小时并进行酶谱分析。将氟化钠 (50-275 ppm F) 添加到孵育缓冲液中。通过向孵育缓冲液中添加 NaF (250-5,000 ppm F) 来测试 NaF 对 MMP-2 和 -9 抑制的可逆性，然后在不存在 F 的情况下进行额外的孵育。F 以剂量响应方式降低唾液和纯化的人 MMP 的前体和活性形式的活性。纯化的明胶酶被 200 ppm F 完全抑制（对于 MMP-2 和 -9，IC50 分别为 100 和 75 ppm F），唾液 MMP-9 被 275 ppm F 完全抑制（IC50 = 200 ppm F）。在 250-1,500 ppm F 时，抑制作用部分可逆，但在 5,000 ppm F 时，抑制作用不可逆。这是第一项描述 NaF 完全抑制 MMP 能力的研究。
The importance of fluoride (F) in preventing dental caries by favorably interfering in the demineralization-remineralization processes is well-established, but its ability to inhibit matrix metalloproteinases (MMPs), which could also help to prevent dentin caries, has not been investigated. This study assessed the ability of F to inhibit salivary and purified human gelatinases MMPs-2 and -9. Saliva was collected from 10 healthy individuals. Pooled saliva was centrifuged, and supernatants were incubated for 1 hr at 37 degrees C and subjected to zymography. Sodium fluoride (50-275 ppm F) was added to the incubation buffer. The reversibility of the inhibition of MMPs-2 and -9 by NaF was tested by the addition of NaF (250-5,000 ppm F) to the incubation buffer, after which an additional incubation was performed in the absence of F. F decreased the activities of pro- and active forms of salivary and purified human MMPs in a dose-response manner. Purified gelatinases were completely inhibited by 200 ppm F (IC50 = 100 and 75 ppm F for MMPs-2 and -9, respectively), and salivary MMP-9 by 275 ppm F (IC50 = 200 ppm F). Inhibition was partially reversible at 250-1,500 ppm F, but was irreversible at 5,000 ppm F. This is the first study to describe the ability of NaF to inhibit MMPs completely.