Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants
Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants
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DOI：
10.1111/j.1365-313x.2011.04687.x
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发表时间：
2011-10-01
期刊：
影响因子：
7.2
通讯作者：
Chen, Shou-Yi
中科院分区：
文献类型：
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作者：
Hao, Yu-Jun;Wei, Wei;Chen, Shou-Yi
NAC转录因子在植物生长，发育和压力反应中起着重要作用。以前，我们在大豆（甘氨酸最大）中鉴定了多个NAC基因。在这里，我们确定了两个基因GMNAC11和GMNAC20在压力反应和其他过程中的作用。这两个基因被多种非生物胁迫和植物激素差异诱导，它们的转录本在根和子叶中很丰富。这两个基因均编码定位于核并结合到核心DNA序列CGT [g/a]的蛋白质。在原生质体测定系统中，GMNAC11充当转录激活剂，而GMNAC20充当轻度抑制器。但是，GMANC20的C末端具有转录激活活性。 GMNAC20的过表达可增强转基因拟南芥植物中的盐和冷冻耐受性；但是，GMNAC11的过表达只能提高盐的耐受性。 GMNAC20的过表达还促进了侧根形成。 GMNAC20可以通过激活DREB/CBF-COR途径来调节胁迫耐受性，并可以通过改变生长素信号相关基因来控制横向根发育。 GMNAC11可能调节Dreb1a和其他与应激相关的基因。在大豆转基因的毛状根中进一步分析了两个GMNAC基因在胁迫耐受性中的作用。这些结果为遗传操作提供了基础，以改善重要作物的农艺性状。
NAC transcription factors play important roles in plant growth, development and stress responses. Previously, we identified multiple NAC genes in soybean (Glycine max). Here, we identify the roles of two genes, GmNAC11 and GmNAC20, in stress responses and other processes. The two genes were differentially induced by multiple abiotic stresses and plant hormones, and their transcripts were abundant in roots and cotyledons. Both genes encoded proteins that localized to the nucleus and bound to the core DNA sequence CGT[G/A]. In the protoplast assay system, GmNAC11 acts as a transcriptional activator, whereas GmNAC20 functions as a mild repressor; however, the C-terminal end of GmANC20 has transcriptional activation activity. Overexpression of GmNAC20 enhances salt and freezing tolerance in transgenic Arabidopsis plants; however, GmNAC11 over-expression only improves salt tolerance. Over-expression of GmNAC20 also promotes lateral root formation. GmNAC20 may regulate stress tolerance through activation of the DREB/CBF-COR pathway, and may control lateral root development by altering auxin signaling-related genes. GmNAC11 probably regulates DREB1A and other stress-related genes. The roles of the two GmNAC genes in stress tolerance were further analyzed in soybean transgenic hairy roots. These results provide a basis for genetic manipulation to improve the agronomic traits of important crops.