Parathyroid hormone-1 receptor down-regulation in kidneys from rats with chronic renal failure
Parathyroid hormone-1 receptor down-regulation in kidneys from rats with chronic renal failure
复制标题
DOI：
10.1159/000056102
复制
发表时间：
2001-01-01
期刊：
影响因子：
3.1
通讯作者：
Brooks, DP
中科院分区：
文献类型：
--
作者：
Edwards, RM;Contino, LC;Brooks, DP
虽然继发性甲状旁腺功能亢进是慢性肾功能衰竭的常见并发症，但很少有研究探讨甲状旁腺激素（PTH）与肾脏的结合特征或慢性肾功能衰竭时PTH受体的调节。使用I-125 PTH相关肽的饱和结合实验的分析（鸡，cPTHrP）显示表观K-d和B-max值分别为1.16 +/- 0.14 nmol/l和338 +/- 22.7 fmol/mg，诱导肾衰竭后3周，PTH-1受体的亲和力没有变化（Kd = 1.51 +/- 0.24 nmol/l），但B-max降低了45%（183 +/- 32.5）。在接受甲状旁腺切除术的正常大鼠中，Kd不变（1.17 +/- 0.09），而B-max增加至459 +/- 31 fmol/mg。我们的结论是慢性肾功能衰竭伴随着肾PTH-1受体的下调。版权所有（C）2001 S.Karger AG，巴塞尔。
Although secondary hyperparathyroidism is a common complication of chronic renal failure, few studies have examined the characteristics of parathyroid hormone (PTH) binding to the kidney or the regulation of the PTH receptor in chronic renal disease, In this study we mea sured PTH binding to the PTH-1 receptor in renal cortical membranes from normal rats and from rats with experimentally induced chronic renal failure, In normal rats, analysis of saturation binding experiments using I-125 PTH-related peptide (chicken, cPTHrP) revealed apparent K-d and B-max values of 1.16 +/- 0.14 nmol/l and 338 +/- 22.7 fmol/mg, respectively, Three weeks following induction of renal failure there was no change in the affinity of the PTH-1 receptor (K-d = 1.51 +/- 0.24 nmol/l) but the B-max was reduced by 45% (183 +/- 32.5). In normal rats which had undergone thyroparathyroidectomy, the Kd was unchanged (1.17 +/- 0.09) while the B-max increased to 459 +/- 31 fmol/mg. We conclude that chronic renal failure is accompanied by a downregulation of renal PTH-1 receptors.Copyright (C) 2001 S.Karger AG, Basel.