Chirality-induced budding: A raft-mediated mechanism for endocytosis and morphology of caveolae?
Chirality-induced budding: A raft-mediated mechanism for endocytosis and morphology of caveolae?
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DOI：
10.1529/biophysj.106.085662
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发表时间：
2007-05-01
影响因子：
3.4
通讯作者：
Rao, Madan
中科院分区：
文献类型：
--
作者：
Sarasij, R. C.;Mayor, Satyajit;Rao, Madan
运输载体（球形囊泡和小管）的形成涉及膜出芽、生长和最终分裂。我们提出了一种膜出芽的机制，其中的倾斜和手性的组成分子，局限于一个补丁的区域A，诱导芽类似于50-100 nm，相当于参与内吞作用的囊泡。由于这种手性和倾斜的脂质分子可能存在于“筏”中，我们建议参与这种机制在产生膜芽的网格蛋白和动力蛋白独立，筏组件介导的内吞作用的糖基磷脂酰肌醇锚定蛋白。我们认为，小窝，永久性的细胞表面结构的特征形态和丰富的筏成分，也可能形成这种机制。因此，分子的手性和倾斜，及其在大空间尺度上的表达可能是一个共同的组织原则，在膜出芽的运输载体。
The formation of transport carriers (spherical vesicles and tubules) involves membrane budding, growth, and ultimately fission. We propose a mechanism of membrane budding, wherein the tilt and chirality of constituent molecules, confined to a patch of area A, induces buds of similar to 50-100 nm that are comparable to vesicles involved in endocytosis. Because such chiral and tilted lipid molecules are likely to exist in "rafts'', we suggest the involvement of this mechanism in generating membrane buds in the clathrin and dynamin-independent, raft-component mediated endocytosis of glycosylphosphatidylinositol-anchored proteins. We argue that caveolae, permanent cell surface structures with characteristic morphology and enriched in raft constituents, are also likely to be formed by this mechanism. Thus, molecular chirality and tilt, and its expression over large spatial scales may be a common organizing principle in membrane budding of transport carriers.