Identification of the Fe–O2 and the Fe=O Heme Species for Indoleamine 2,3-Dioxygenase during Catalytic Turnover
Identification of the Fe–O2 and the Fe=O Heme Species for Indoleamine 2,3-Dioxygenase during Catalytic Turnover
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DOI：
10.1246/cl.2010.36
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发表时间：
2010-01
影响因子：
1.6
通讯作者：
S. Yanagisawa;Keiko Yotsuya;Yumi Hashiwaki;M. Horitani;H. Sugimoto;Y. Shiro;E. Appelman;T. Ogura
中科院分区：
文献类型：
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作者：
S. Yanagisawa;Keiko Yotsuya;Yumi Hashiwaki;M. Horitani;H. Sugimoto;Y. Shiro;E. Appelman;T. Ogura
共振拉曼光谱已被应用到两个不同的时间物种的吲哚胺2，3-双加氧酶在催化营业额。在569和798 cm-1处分别发现了两个氧同位素敏感的拉曼峰。798 cm-1谱带的16 O 18 O分析表明存在铁氧血红素，这与以前提出的反应机理不一致.因此，本研究为可能的反应中间体的结构提供了物理基础。
Resonance Raman spectroscopy has been applied to two distinct temporal species of indoleamine 2,3-dioxygenase during catalytic turnover. We have identified two oxygen-isotope-sensitive Raman modes at 569 and 798cm -1 for the two respective species. The 16 O 18 O analysis of the 798cm -1 band indicates the existence of a ferryl-oxo heme, which is inconsistent with the previously proposed reaction mechanism. The present study thus provides a physical basis for the structures of the possible reaction intermediates.