Histological Characterization of Resistance to Different Root-Knot Nematode Species Related to Phenolics Accumulation in Capsicum annuum.
Histological Characterization of Resistance to Different Root-Knot Nematode Species Related to Phenolics Accumulation in Capsicum annuum.
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DOI：
10.1094/phyto-95-0158
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发表时间：
2005-02
期刊：
影响因子：
3.2
通讯作者：
A. Pegard;G. Brizzard;A. Fazari;O. Soucaze;P. Abad;C. Djian-Caporalino
中科院分区：
文献类型：
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作者：
A. Pegard;G. Brizzard;A. Fazari;O. Soucaze;P. Abad;C. Djian-Caporalino
辣椒CM334是辣椒抗疫霉的一个抗病品种。和马铃薯Y病毒属（Potyviruses），抗性基因完全抑制了根结线虫（Meloidogyne spp.）的繁殖。目前的研究比较了该抗性品系和感病品种对三种最具破坏性的根结线虫感染的组织学反应：M。arenaria，M. incognita或M. javanica。CM 334对根结线虫的抗性与限制线虫穿透的未鉴定因素以及穿透后的生化反应（包括过敏反应）有关，过敏反应显然阻止了线虫的迁移，从而阻止了幼虫的发育和繁殖。高效液相色谱分析表明，酚类化合物，特别是绿原酸，可能参与CM334抗性。在抗线感染的反应不同的根结线虫物种和相关的线虫行为和致病性在敏感品种：线虫物种，迅速达到维管圆柱体和启动的取食位点在敏感品种迅速识别CM334和停止在表皮或皮层。通过与其他抗虫品系的比较，我们认为，抗虫反应的发生时间和抗性机制因植株基因型、抗性基因和根结线虫种类而异。
ABSTRACT In the pepper Capsicum annuum CM334, which is used by breeders as a source of resistance to Phytophthora spp. and potyviruses, a resistance gene entirely suppresses reproduction of the root-knot nematode (Meloidogyne spp.). The current study compared the histological responses of this resistant line and a susceptible cultivar to infection with the three most damaging root-knot nematodes: M. arenaria, M. incognita, or M. javanica. Resistance of CM334 to root-knot nematodes was associated with unidentified factors that limited nematode penetration and with post-penetration biochemical responses, including the hypersensitive response, which apparently blocked nematode migration and thereby prevented juvenile development and reproduction. High-performance liquid chromatography analysis suggested that phenolic compounds, especially chlorogenic acid, may be involved in CM334 resistance. The response to infection in the resistant line varied with root-knot nematode species and was correlated with nematode behavior and pathogenicity in the susceptible cultivar: nematode species that quickly reached the vascular cylinder and initiated feeding sites in the susceptible cultivar were quickly recognized in CM334 and stopped in the epidermis or cortex. After comparing our data with those from other resistant pepper lines, we suggest that timing of the resistance response and the mechanism of resistance vary with plant genotype, resistance gene, and root-knot nematode species.