Interleukin 1 Production and Accessory Cell Function of Rat Alveolar Macrophages Exposed to Mineral Dust Particles
Interleukin 1 Production and Accessory Cell Function of Rat Alveolar Macrophages Exposed to Mineral Dust Particles
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暴露于矿物粉尘颗粒的大鼠肺泡巨噬细胞的白介素 1 产生和辅助细胞功能
DOI：
10.1111/j.1348-0421.1987.tb03090.x
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发表时间：
1987
影响因子：
2.6
通讯作者：
Y. Kubota
中科院分区：
文献类型：
--
作者：
Y. Oghiso;Y. Kubota
本文研究了可吸入矿物粉尘对肺泡巨噬细胞（AM）和T淋巴细胞的免疫刺激作用。当大鼠AM与纤维化二氧化硅和石棉颗粒一起孵育时，培养上清液中产生了显著的白细胞介素1（IL-1）活性，而单独的AM或与非纤维化二氧化钛（TiO 2）颗粒一起孵育的AM都没有产生可检测量的IL-1。使用IL-2依赖性CTLL-2测定法检测的白细胞介素2（IL-2）活性在所有这些培养物中均未检出。还显示，伴刀豆球蛋白A诱导的T淋巴细胞增殖在仅与纤维化颗粒孵育而非与非纤维化颗粒孵育的自体AM和尼龙羊毛通过的脾细胞共培养物中增强。仅在暴露于纤维化颗粒的共培养物中检测到较高的IL-1活性，而IL-2活性在这些共培养物中几乎相似。这些结果表明，在不同的矿物粉尘颗粒对肺巨噬细胞和T淋巴细胞的免疫刺激作用的差异。
Immunostimulatory effects of respirable mineral dust particles on alveolar macrophages (AM) and T lymphocytes were tested in vitro. When rat AM were incubated with fibrogenic silica and asbestos particles, a significant interleukin 1 (IL‐1) activity was generated into the culture supernatants, whereas neither AM alone nor AM incubated with non‐fibrogenic titanium dioxide (TiO2) particles, however, produced a detectable amount of IL‐1. Interleukin 2 (IL‐2) activity, as tested with IL‐2‐dependent CTLL‐2 assays, was not detectable from all of these cultures. It was also revealed that concanavalin A‐induced proliferation of T lymphocytes was enhanced in autologous AM and nylon wool‐passed spleen cell co‐cultures incubated only with fibrogenic particles, but not with non‐fibrogenic particles. Higher IL‐1 activity was detected only from co‐cultures exposed to fibrogenic particles, whereas IL‐2 activity was almost similar among these co‐cultures. These results indicate the differences in immunostimulatory effects on pulmonary macrophages and T lymphocytes among a variety of mineral dust particles.
人巨噬细胞支持 T 细胞增殖不需要分泌白细胞介素 1。
影响因子：
4.3
作者：
Haq,AU;Mayernik,DG;Orosz,C;Rinehart,JJ
通讯作者：
Rinehart,JJ