Expression of the helix-loop-helix factor Id during mouse embryonic development.
Expression of the helix-loop-helix factor Id during mouse embryonic development.
复制标题
小鼠胚胎发育期间螺旋-环-螺旋因子Id的表达。
DOI：
10.1006/dbio.1993.1258
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
O'Brien,TX
中科院分区：
文献类型：
--
作者：
Evans,SM;O'Brien,TX
心脏细胞中螺旋-环-螺旋（HLH）蛋白Id的表达和调控及其对心脏基因表达的负面影响表明，Id可能在心脏发育中发挥作用。为了进一步研究这一问题，我们对发育中的小鼠胚胎（从胚胎第7天到15.5天）和新生儿心脏中的Id mRNA进行了状况分析。与其作为分化抑制剂的假设作用相一致，Id mRNA在增殖的间充质和上皮组织的特定群体中表达，其表达随着分化的进展而下降。此外，Id mRNA的杂交模式与HLH和homeobox家族的另外两个发育重要基因twist和hox 7/7.1的杂交模式有很大的重叠。在早期上皮小体或小体的肌瘤室中未观察到Id mRNA杂交，这表明Id不太可能在这些早期肌源性前体中发挥抑制作用。mRNA在发育中的神经系统的特定区域表达。在心脏中，Id mRNA在发育中的心垫和流出道嵴中高水平表达，并在新生儿心脏瓣膜组织中继续高水平表达。
Expression and regulation of the helix-loop-helix (HLH) protein Id in cardiac cells and its negative effect on cardiac gene expression indicate that Id may play a role in cardiac development. To investigate this issue further, we have performedin situanalysis of Id mRNA in developing mouse embryos from Embryonic Days 7 to 15.5 and in neonatal heart. Consistent with its postulated role as an inhibitor of differentiation, Id mRNA was expressed in specific populations of proliferating mesenchymal and epithelial tissues, with its expression declining as differentiation progressed. In addition, patterns of Id mRNA hybridization overlapped considerably with those of two other developmentally important genes, twist and hox 7/7.1, of the HLH and homeobox family, respectively. Id mRNA hybridization was not observed in early epithelial somites or in the myotomal compartment of the somite, suggesting that Id is unlikely to play an inhibitory role in these early myogenic precursors. Id mRNA was expressed in specific regions of the developing nervous system. In the heart, Id mRNA was expressed at high levels in developing cardiac cushions and ridges of the outflow tract and continued to be expressed at high levels in valvular tissue of the neonatal heart.