Convergent Loss of Awn in Two Cultivated Rice Species Oryza sativa and Oryza glaberrima Is Caused by Mutations in Different Loci.
Convergent Loss of Awn in Two Cultivated Rice Species Oryza sativa and Oryza glaberrima Is Caused by Mutations in Different Loci.
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DOI：
10.1534/g3.115.020834
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发表时间：
2015-09-02
期刊：
影响因子：
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通讯作者：
Ashikari M
中科院分区：
文献类型：
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作者：
Furuta T;Komeda N;Asano K;Uehara K;Gamuyao R;Angeles-Shim RB;Nagai K;Doi K;Wang DR;Yasui H;Yoshimura A;Wu J;McCouch SR;Ashikari M
长芒是野生稻的显著形态特征之一。这个器官被认为有助于种子传播和防止动物捕食。然而，大多数栽培品种的米（Oryza sativa）和光谷米（Oryza glaberrima）已经失去了形成长芒的能力。导致这两种水稻失去芒的遗传因素在很大程度上仍然未知。在这里，我们评估了三组染色体片段代换系（CSSLs）在一个共同的水稻遗传背景(cv。（Koshihikari），这些基因片段来自于Oryza nivara、Oryza rufipogon和glaberrima捐赠者。对这些文库的表型分析表明，栽培水稻中存在3个基因，分别是修长调节因子1 （RAE1）、RAE2和RAE3，这些基因参与了修长水稻的修长。其中两个基因RAE1和RAE2的供体片段在CSSLs中诱导长芒形成，而在glaberrima中，sativa的RAE3片段诱导长芒形成。这些结果表明，两种栽培水稻品种，即黄稻和光稻，已经走上了独立的无芒之路。
A long awn is one of the distinct morphological features of wild rice species. This organ is thought to aid in seed dispersal and prevent predation by animals. Most cultivated varieties of Oryza sativa and Oryza glaberrima, however, have lost the ability to form long awns. The causal genetic factors responsible for the loss of awn in these two rice species remain largely unknown. Here, we evaluated three sets of chromosome segment substitution lines (CSSLs) in a common O. sativa genetic background (cv. Koshihikari) that harbor genomic fragments from Oryza nivara, Oryza rufipogon, and Oryza glaberrima donors. Phenotypic analyses of these libraries revealed the existence of three genes, Regulator of Awn Elongation 1 (RAE1), RAE2, and RAE3, involved in the loss of long awns in cultivated rice. Donor segments at two of these genes, RAE1 and RAE2, induced long awn formation in the CSSLs whereas an O. sativa segment at RAE3 induced long awn formation in O. glaberrima. These results suggest that the two cultivated rice species, O. sativa and O. glaberrima, have taken independent paths to become awnless.