Plant DNA barcodes, taxonomic management, and species discovery in tropical forests.
Plant DNA barcodes, taxonomic management, and species discovery in tropical forests.
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DOI：
10.1007/978-1-61779-591-6_18
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Webb, Campbell O
中科院分区：
文献类型：
--
作者：
Dick, Christopher W;Webb, Campbell O
DNA 条形码在热带森林的物种识别和分类学发现方面具有巨大潜力。 DNA 条形码的这种使用需要已知分类群的参考 DNA 文库，用于匹配来自未鉴定样本的 DNA。在更基本的层面上，它预设区域物种库中的物种具有拉丁二项式。但在物种丰富的热带森林中情况并非如此，其中许多物种对于科学来说是新物种，或者是界限不明确的物种复合体的成员。本章描述了面向分类发现的工作流程，其中包括新物种、分布记录和杂交形式的发现，以及森林清查地块中分类实体的管理。它概述了实验室技术人员、野外工作人员和植物标本室分类学家的角色，最后讨论了用于识别最近进化进化枝中物种的潜在多位点核 DNA 方法。
DNA barcodes have great potential for species identification and taxonomic discovery in tropical forests. This use of DNA barcodes requires a reference DNA library of known taxa with which to match DNA from unidentified specimens. At an even more basic level, it presupposes that the species in the regional species pool have Latin binomials. This is not the case in species-rich tropical forests in which many species are new to science or members of poorly circumscribed species complexes. This chapter describes a workflow geared toward taxonomic discovery, which includes the discovery of new species, distribution records, and hybrid forms, and to management of taxonomic entities in forest inventory plots. It outlines the roles of laboratory technicians, field workers and herbarium-based taxonomists, and concludes with a discussion of potential multilocus nuclear DNA approaches for identifying species in recently evolved clades.