MOLECULAR-CLONING OF THE GENOME OF A CARDIOTROPIC COXSACKIE-B3 VIRUS - FULL-LENGTH REVERSE-TRANSCRIBED RECOMBINANT CDNA GENERATES INFECTIOUS VIRUS IN MAMMALIAN-CELLS
MOLECULAR-CLONING OF THE GENOME OF A CARDIOTROPIC COXSACKIE-B3 VIRUS - FULL-LENGTH REVERSE-TRANSCRIBED RECOMBINANT CDNA GENERATES INFECTIOUS VIRUS IN MAMMALIAN-CELLS
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DOI：
10.1073/pnas.82.14.4818
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
HOFSCHNEIDER, PH
中科院分区：
文献类型：
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作者：
KANDOLF, R;HOFSCHNEIDER, PH
本文报道了从嗜心性科萨基B3病毒（Nancy株）的单链RNA基因组合成的双链cDNA（互补DNA）的分子克隆。在将重组质粒DNA转染到哺乳动物细胞中时，全长约7500个核苷酸的逆转录克隆病毒cDNA产生感染性抗原性相同的科萨基B3病毒，证明了生物活性病毒cDNA拷贝的分子克隆。克隆的cDNA通过限制性内切酶分析和5“端的部分核苷酸测序来表征。所描述的科萨基B3病毒cDNA现已可用于研究人类肠道病毒性心脏病的分子基础，并为疑似病毒性心脏病患者提供有价值的诊断手段。
The molecular cloning of double-stranded cDNA (complementary DNA) synthesized from the single-stranded RNA genome of the cardiotropic Coxsackie B3 virus (Nancy strain) is reported. Full-length reverse-transcribed cloned viral cDNA of .apprxeq.7500 nucleotides generated infectious antigenically identical Coxsackie B3 virus upon transfection of recombinant plasmid DNA into mammalian cells, demonstrating the molecular cloning of a biologically active viral cDNA copy. The cloned cDNA is characterized by restriction enzyme analysis and partial nucleotide sequencing of the 5'' end. The Coxsackie B3 virus cDNA described can now be used to study the molecular basis of human enteroviral heart disease, and it provides a valuable diagnostic means for patients with suspected viral heart disease.