First detection of porcine circovirus type 3 on commercial pig farms in Poland
First detection of porcine circovirus type 3 on commercial pig farms in Poland
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DOI：
10.1111/tbed.12672
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发表时间：
2017-10-01
影响因子：
4.3
通讯作者：
Biernacka, K.
中科院分区：
文献类型：
--
作者：
Stadejek, T.;Wozniak, A.;Biernacka, K.
猪圆环病毒3型（Porcine Circovirus type 3，PCV 3）是近年来在美国和中国发现的一种新型圆环病毒，可引起猪皮炎肾病综合征、繁殖障碍、呼吸系统疾病和多系统炎症。这项研究报告了欧洲首次在波兰农场猪血清中鉴定出PCV 3。2014年至2017年期间，从代表波兰不同地区、不同规模和健康状况的14个商业农场的母猪和3- 20周龄猪中共采集了1,050份血清样本。按4-6份合并样本，并采用实时PCR检测PCV 3。14个猪场中有12个检出PCV 3 DNA，检出率为85.7%。在PCV 3阳性农场中，在5.9%至65%的血清池中检出病毒。PCV 3在断奶仔猪和肥育猪中最常见（分别占血清池的26.1%和28.0%）。ORF 2基因359个核苷酸的序列分析表明，与美国PCV 3-US/SD 2016同源性最高，为99.7%。我们的研究结果表明，PCV 3是波兰猪中的一种常见病毒，但与不明原因的疾病没有联系。
Porcine circovirus type 3 (PCV3) is a novel circovirus species recently discovered in USA and China in cases of porcine dermatitis and nephropathy syndrome, reproductive failure, respiratory disease and multisystemic inflammation. This study reports on the first identification of PCV3 in Europe, in serum from pigs from Polish farms. A total of 1,050 serum samples were collected between 2014 and 2017 from sows and 3-20weeks old pigs from 14 commercial farms representing different regions of Poland, different size and health status. The samples were pooled by 4-6 and tested with real-time PCR for PCV3. PCV3 DNA was detected in 12 of 14 farms (85.7%). On the PCV3-positive farms, the virus was detected in 5.9% to 65% serum pools. PCV3 was most common among weaned pigs and finishers (26.1% and 28.0% of serum pools, respectively). Sequence analysis of 359 nucleotide fragment of ORF2 showed highest identity of 99.7% to PCV3-US/SD2016 from USA. Our results indicate that PCV3 is a common virus among Polish pigs but no links to unexplained disease conditions were established.