Genetic classification of "Sapporo-like viruses"
Genetic classification of "Sapporo-like viruses"
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DOI：
10.1007/s007050170024
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Aymard, M
中科院分区：
文献类型：
--
作者：
Schuffenecker, I;Ando, T;Aymard, M
“札幌样病毒”（SLV）和“诺瓦克样病毒”（NLV）是人类急性胃肠炎的重要原因。虽然NLV在遗传学上被分为三个主要的遗传组，包括17个遗传亚组，但SLV在可比遗传组中的分类仍有待确定。为了对SLV和NLV进行统一分类，对从法国婴儿中新检测到的2株SLV毒株的序列与已发表的9株SLV和19株NLV毒株的序列进行了分析。对衣壳蛋白基因、RNA聚合酶基因、3'开放阅读框（3' ORF）、与衣壳蛋白基因重叠的开放阅读框（ORF）和3'非翻译区（3' UTR）的序列进行了距离分析和系统发育分析。显示成对距离的频率分布的直方图和系统发育树的拓扑结构表明，SLV和NLV可以统一地基于整个衣壳序列进行分类，并且I I SLV株可以在遗传上分为3个主要遗传群，基因群I、II和III，包括5个遗传亚群。在4个剩余的基因组区域中也保持了II SLV毒株分化成这些遗传组，而RNA聚合酶和衣壳基因之间的连接处的序列显示为基因组特异性的。
"Sapporo-like viruses" (SLVs) and "Norwalk-like viruses"' (NLVs) are an important cause of acute gastroenteritis in humans. While NLVs have been genetically classified into three major genetic groups consisting of 17 genetic subgroups, a classification of SLVs into comparable genetic groups remains to be determined. In an attempt to classify both SLVs and NLVs uniformly, the sequences of 2 SLV strains newly detected from French infants were analysed together with the published sequences of 9 SLV and 19 NLV strains. Distance and phylogenetic analyses were conducted on the sequences of the capsid gene, RNA polymerase gene, 3' open reading frame (3'ORF), ORF overlapping the capsid gene, and 3' untranslated region (3'UTR). The histogram showing frequency distribution of pairwise distances and the topology of the phylogenetic tree demonstrated that SLVs and NLVs could be classified uniformly on the basis of the entire capsid sequences and that the I I SLV strains could be genetically classified into 3 major genetic groups, genogroups I, II and III, comprised of 5 genetic subgroups. The differentiation of the I I SLV strains into these genetic groups was also maintained in the 4 remaining genome regions, while the sequences at the junction between the RNA polymerase and capsid genes were shown to be genogroup-specific.