Emergence of Japanese encephalitis virus genotype V in the Republic of Korea.
Emergence of Japanese encephalitis virus genotype V in the Republic of Korea.
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DOI：
10.1186/1743-422x-8-449
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发表时间：
2011-09-23
期刊：
影响因子：
4.8
通讯作者：
Evans BP
中科院分区：
文献类型：
--
作者：
Takhampunya R;Kim HC;Tippayachai B;Kengluecha A;Klein TA;Lee WJ;Grieco J;Evans BP
2009年，流行性乙型脑炎病毒V型病毒在离开亚洲大陆57年后再次出现在亚洲(中国)，从而强调需要加强区域监测工作。对19个JEV阳性蚊池(18个三带喙库蚊和1个CX蚊池)进行了基因分型。2008年至2010年，从韩国各地不同地理位置收集的阳性池中收集了三种不同的抗体。从19个池中对JEV基因组的两个区域进行了测序：包膜基因和非结构蛋白5(NS5)/3‘-非翻译区(UTR)。十八池三带喙库蚊和一池CX。Bitaeniorhynchus的I型和V型分别为阳性。华支睾吸虫E基因全序列比对Biaeniorhynchus与Muar株有很高的氨基酸相似性(98.8%)，Muar株是1952年从马来西亚脑炎患者中分离到的第一个V型报告菌株。这项研究是韩国首次报道乙型脑炎病毒V型。V型基因在亚洲(中国和韩国)半个多世纪后的重新出现及其在CX的发现。Bitaeniorhynchus是一种以前在韩国未知携带乙脑病毒的蚊子物种，它强调需要加强乙脑监测，以监测该区域内乙脑病毒毒株的动态。未来的研究结果可能会对乙脑疫苗接种/预防战略产生影响。
Japanese encephalitis virus (JEV) genotype V reemerged in Asia (China) in 2009 after a 57-year hiatus from the continent, thereby emphasizing a need to increase regional surveillance efforts. Genotypic characterization was performed on 19 JEV-positive mosquito pools (18 pools of Culex tritaeniorhynchus and 1 pool of Cx. bitaeniorhynchus) from a total of 64 positive pools collected from geographically different locations throughout the Republic of Korea (ROK) during 2008 and 2010. Two regions of the JEV genome were sequenced from 19 pools; the envelope gene and the nonstructural protein 5 (NS5)/3'-untranslated region (UTR). Eighteen pools of Culex tritaeniorhynchus and one pool of Cx. bitaeniorhynchus were positive for genotype I and genotype V, respectively. Sequence alignment of the complete E gene from Cx. bitaeniorhynchus showed high amino acid similarity (98.8%) to the Muar strain, characterized as the first report of genotype V, isolated from an encephalitis patient in Malaysia in 1952. This study represents the first report of JEV genotype V in the ROK. The reemergence of genotype V in Asia (China and ROK) after more than a half-century and its discovery in Cx. bitaeniorhynchus, a mosquito species previously unknown to carry JEV in the ROK, emphasizes the need for enhanced JE surveillance to monitor the dynamics of JEV strains within the region. Future findings may have implications with regard to JEV vaccination/prevention strategies.