INTRACELLULAR-LOCALIZATION OF VIRAL POLYPEPTIDES DURING SIMIAN VIRUS-40 INFECTION
INTRACELLULAR-LOCALIZATION OF VIRAL POLYPEPTIDES DURING SIMIAN VIRUS-40 INFECTION
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DOI：
10.1128/jvi.32.2.648-660.1979
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
NEHORAYAN, A
中科院分区：
文献类型：
--
作者：
KASAMATSU, H;NEHORAYAN, A
通过免疫荧光技术分析被 SV-40 感染的非洲绿猴肾细胞的性质和感染过程中病毒多肽出现的时间过程。研究中使用的试剂为抗Vp1血清和亲和纯化的抗Vp1免疫球蛋白G、抗Vp3血清、抗病毒（抗V）血清和抗肿瘤抗原血清。结果总结如下。在与抗Vp1抗体反应的感染细胞中观察到三种类型的染色：核染色、核周染色和核周伴随细胞质染色。在感染后期，在受感染细胞的细胞核周围也观察到高度结构化的染色。在与抗 Vp3 血清反应时，在整个感染过程中染色仅限于细胞核内。 Vp1 和 Vp3 抗原似乎占据细胞核区域的不同空间区域。 Vp1和Vp3抗原在感染过程中同时表达。感染早期观察到的中心粒染色与肿瘤 (T-) 抗原的出现平行，直到感染后 24 小时，此后随着感染的进展，中心粒染色阳性的频率减少。 T抗原在感染后约8小时首次表达，Vp1和Vp3抗原在感染后约20小时首次表达。
African green monkey kidney cells infected by SV-40 were analyzed by immunofluorescence techniques for the nature and the time course of the appearance of viral polypeptides during infection. Reagents used in the study were anti-Vp1 sera and affinity-purified anti-Vp1 immunoglobulin G, anti-Vp3 sera, antivirus (anti-V) sera and anti-tumor antigen sera. The results are summarized as follows. Three types of staining, nuclear, perinuclear and perinuclear accompanied by cytoplasmic staining, were observed in infected cells in reaction with anti-Vp1 antibody. A highly structured staining was also observed at the periphery of nuclei of infected cells late in infection. In reaction with anti-Vp3 serum, the staining was confined within nuclei of cells throughout infection. Vp1 and Vp3 antigens seem to occupy different spacial regions of the nuclear area in cells. Vp1 and Vp3 antigens were expressed simultaneously during infection. Centriolar staining observed early in infection paralleled the appearance of tumor (T-) antigen until 24 h after infection, after which time the frequency of positive centriolar staining decreased as infection progressed. T-antigen was first expressed at about 8 h after infection, and Vp1 and Vp3 antigens were first expressed at about 20 h after infection.