Implication of signaling pathways involving calcium, phosphorylation and active oxygen species in methyl jasmonate-induced defense responses in grapevine cell cultures
Implication of signaling pathways involving calcium, phosphorylation and active oxygen species in methyl jasmonate-induced defense responses in grapevine cell cultures
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DOI：
10.1016/j.jplph.2009.05.015
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Merillon, Jean-Michel
中科院分区：
文献类型：
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作者：
Faurie, Bertrand;Cluzet, Stephanie;Merillon, Jean-Michel
诱导子的感知通过多种早期信号转导途径触发植物防御反应。茉莉酸甲酯（MeJA）刺激葡萄藤（葡萄属葡萄）的防御反应。我们研究了参与各种合作伙伴（钙，活性氧，可逆磷酸化）在MeJA诱导的反应，通过使用药理学方法。我们使用了特定的钙通道效应物和丝氨酸/苏氨酸磷酸酶、超氧化物歧化酶和NAD（P）H氧化酶的抑制剂，并研究了葡萄藤植物抗毒素二苯乙烯（白藜芦醇及其葡萄糖苷，白藜芦醇苷，主要形式）的产生。RNA积累的两个基因编码酶参与芪合成（PAL和STS），三个基因编码的发病相关蛋白（CHIT 4C，PIN和GLU）和一个基因编码的酶产生茉莉酸酯（LOX）进行了评估。钙和它的来源似乎发挥了主要的死记硬背MeJA诱导葡萄藤防御反应。如果钙离子从流入质膜被抑制，植物抗毒素生产受到强烈影响，而钙离子从细胞内室似乎没有参与。ROS的产生似乎干扰MeJA刺激的防御反应，蛋白质磷酸化/去磷酸化事件也发挥了直接的作用。(C)2009年Elsevier GmbH。All rights reserved.
Perception of elicitors triggers plant defense responses via various early signal transduction pathways. Methyl jasmonate (MeJA) stimulates defense responses in grapevine (Vitis vinifera). We investigated the involvement of various partners (calcium, ROS, reversible phosphorylation) in MeJA-induced responses by using a pharmacological approach. We used specific calcium channel effectors and inhibitors of serine/threonine phosphatases, superoxide dismutase and NAD(P)H oxidase and investigated production of stilbenes (resveratrol and its glucoside, piceid, the major form), which are the grapevine phytoalexins. RNA accumulation of two genes encoding enzymes involved in stilbene synthesis (PAL and STS), three genes encoding pathogenesis-related proteins (CHIT4C, PIN and GLU) and one gene encoding an enzyme producing jasmonates (LOX) were also assessed. Calcium and its origin seemed to play a major rote in MeJA-induced grapevine defense responses. Phytoalexin production was strongly affected if calcium from the influx plasma membrane was inhibited, whereas calcium from the intracellular compartments did not seem to be involved. ROS production seemed to interfere with MeJA-stimulated defense responses, and protein phosphorylation/dephosphorylation events also played a direct rote. (C) 2009 Elsevier GmbH. All rights reserved.