SEQUENCE-ANALYSIS OF CLONED CDNA FOR RAT SUBSTANCE-P PRECURSOR - EXISTENCE OF A 3RD SUBSTANCE-P PRECURSOR
SEQUENCE-ANALYSIS OF CLONED CDNA FOR RAT SUBSTANCE-P PRECURSOR - EXISTENCE OF A 3RD SUBSTANCE-P PRECURSOR
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DOI：
10.1016/s0006-291x(86)80282-0
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发表时间：
1986-09-30
影响因子：
3.1
通讯作者：
NAKANISHI, S
中科院分区：
文献类型：
--
作者：
KAWAGUCHI, Y;HOSHIMARU, M;NAKANISHI, S
The sequence of the mRNA for the rat substance P precursor (preprotachykinin A) has been elucidated by molecular cloning and sequence analysis. The deduced amino acid sequence of rat preprotachykinin A indicates that it contains both substance P and substance K but differs in the sequence organization from either bovine .alpha.- or .beta.-preprotachykinin A reported previously. The existence of the bovine mRNA for the third preprotachykinin A has thus been examined and evidenced by the isolation of the corresponding cDNA clone. This mRNA, named .gamma.-preprotachykinin A mRNA, deletes the sequence precisely corresponding to the exon 4 sequence of the preprotachykinin A gene. Thus, alternative RNA splicing in the expression of the single preprotachykinin A gene results in the generation of three different forms of the preprotachykinin A mRNAs.
