A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy
A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy
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DOI：
10.1016/j.bbrc.2008.09.097
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发表时间：
2008-12-05
影响因子：
3.1
通讯作者：
Ogura, Toshihiko
中科院分区：
文献类型：
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作者：
Ogura, Toshihiko
扫描电子显微镜（SEM）中生物样品的对比度很弱。为了通过SEM检查生物样品，需要许多步骤和/或特殊设备来制备样品。在这里，我们描述了一种方法，使用未染色的生物样品下的40纳米碳膜，以给出一个高对比度的图像，其中的图像是由二次电子（SE）信号在低加速电压，1.5千伏的e检测。在这些条件下，很难检测到生物样本前方的直接信号。高对比度图像是由SE从薄碳膜的下表面产生的。因此，电子束对样品的损伤非常低。我们的方法可以用来观察各种细菌、病毒等生物样品。和蛋白质复合物。(C)2008年爱思唯尔公司All rights reserved.
The contrast of biological samples in scanning electron microscopy (SEM) is very weak. To examine a biological specimen by SEM, many steps and/or special equipment are required to prepare the sample. Here, we describe a method using an Unstained biological sample Under a 40 nm carbon film to give a high contrast image, where the image is detected by the secondary electron (SE) signal at a low accelerating volt,e of 1.5 kV. Under these conditions, it is hard to detect a direct signal front a biological specimen. The age high contrast image is created by the SEs from the lower surface of the thin carbon film. Therefore, the damage to the sample from the electron beam is very low. Our method can be utilized to observe various biological samples of bacteria, viruses. and protein complexes. (C) 2008 Elsevier Inc. All rights reserved.