Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative.
Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative.
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DOI：
10.1016/s0002-9394(14)71130-4
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发表时间：
1997-06
影响因子：
4.2
通讯作者：
R. Y. Kim;L. Hu;T. Flotte;E. Gragoudas;L. Young
中科院分区：
文献类型：
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作者：
R. Y. Kim;L. Hu;T. Flotte;E. Gragoudas;L. Young
目的检查苯并卟啉衍生物血管造影作为研究实验性脉络膜黑色素瘤中光敏剂生物分布的一种方式。方法本研究使用苯并卟啉衍生物的脂质体制剂。使用 Topcon ImageNet H1024 数字成像系统、Kodak Megaplus 摄像机和 Topcon TRC-50-VT 眼底相机对 10 只兔子（6 只用于实验性脉络膜黑色素瘤，2 只用于正常脉络膜，2 只用于虹膜）进行数字苯并卟啉衍生物血管造影。每只兔子只使用一只眼睛。使用专为苯并卟啉衍生物设计的滤光片（峰值吸收在 580 nm 处，峰值发射在 695 nm 处）。将苯并卟啉衍生物 (1 mg/kg) 注射到耳静脉中，同时获取肿瘤、正常脉络膜或虹膜的图像。在注射后的前 3 小时和 24 小时内获得后续图像。使用 1 mg/kg 苯并卟啉衍生物对所有 10 只兔子进行荧光显微镜检查。在进行血管造影的同时，将荷瘤眼摘除，比较两组结果的最大染料积累。结果数字血管造影显示，注射后15至45分钟，肿瘤中出现最大苯并卟啉衍生物荧光。荧光光度测定证实了这些结果。结论光敏剂血管造影是确定光动力治疗最佳治疗时间的有效方式。
PurposeTo examine benzoporphyrin derivative angiography as a modality for studying photosensitizer biodistribution in experimental choroidal melanomas.MethodsA liposomal preparation of benzoporphyrin derivative was used in this study. Digital benzoporphyrin derivative angiograms were performed in 10 rabbits (six for experimental choroidal melanomas, two for normal choroids, and two for irides) using a Topcon ImageNet H1024 digital imaging system, a Kodak Megaplus video camera, and a Topcon TRC-50-VT fundus camera. Only one eye from each rabbit was used. Filters specifically designed for benzoporphyrin derivative (peak absorption at 580 nm and peak emission at 695 nm) were used. Benzoporphyrin derivative (1 mg/kg) was injected into an ear vein while images of tumor, normal choroid, or iris were being obtained. Follow-up images were obtained during the first 3 hours and at 24 hours after injection. Fluorescence microscopy was performed in all 10 rabbits using 1 mg/kg of benzoporphyrin derivative. Tumor-bearing eyes were enucleated at the same time points that angiograms were performed, and the two sets of results were compared for maximum dye accumulation.ResultsDigital angiography demonstrated that maximal benzoporphyrin derivative fluorescence occurred in tumors 15 to 45 minutes after injection. Fluorescence photometry corroborated these results.ConclusionPhotosensitizer angiography is a valid modality for determining the optimum treatment time for photodynamic therapy.