Acetylcholine affects osteocytic MLO-Y4 cells via acetylcholine receptors
Acetylcholine affects osteocytic MLO-Y4 cells via acetylcholine receptors
复制标题
乙酰胆碱通过乙酰胆碱受体影响骨细胞 MLO-Y4 细胞
DOI：
10.1016/j.mce.2014.01.021
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发表时间：
2014-03-25
影响因子：
4.1
通讯作者：
Wang, Hang
中科院分区：
文献类型：
--
作者：
Ma, Yuanyuan;Li, Xianxian;Wang, Hang
识别神经元对骨重建的控制已成为骨生物学许多重要进展之一。胆碱能活动最近被证明通过复杂的细胞调节网络有利于骨量的增加。在这里，我们鉴定了M受体和烟碱型乙酰胆碱受体(m-和nAChRs)在小鼠胫骨组织和成骨细胞MLO-Y4中的基因表达。乙酰胆碱是一种经典的神经递质和骨神经介质，它不仅影响AChR亚单位的mRNA表达，而且显著地诱导骨细胞的增殖和活性。此外，乙酰胆碱可通过乙酰胆碱受体相互调节RANKL和OPG的mRNA表达，使骨细胞RANKL/OPG的mRNA比值显著增加。在MLO-Y4细胞中神经肽Y和神经肽Reelin的表达也受乙酰胆碱通过m-和nAChRs调节。这些结果表明，骨细胞型乙酰胆碱受体可能是一种新的有价值的细胞功能甚至骨重建的介体。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
The identification of the neuronal control of bone remodeling has become one of the many significant recent advances in bone biology. Cholinergic activity has recently been shown to favor bone mass accrual by complex cellular regulatory networks. Here, we identified the gene expression of the muscarinic and nicotinic acetylcholine receptors (m- and nAChRs) in mice tibia tissue and in osteocytic MLO-Y4 cells. Acetylcholine, which is a classical neurotransmitter and an osteo-neuromediator, not only influences the mRNA expression of the AChR subunits but also significantly induces the proliferation and viability of osteocytes. Moreover, acetylcholine treatment caused the reciprocal regulation of RANKL and OPG mRNA expression, which resulted in a significant increase in the mRNA ratio of RANKL:OPG in osteocytes via acetylcholine receptors. The expression of neuropeptide Y and reelin, which are two neurogenic markers, was also modulated by acetylcholine via m- and nAChRs in MLO-Y4 cells. These results indicated that osteocytic acetylcholine receptors might be a new valuable mediator for cell functions and even for bone remodeling. (C) 2014 Elsevier Ireland Ltd. All rights reserved.