Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5.
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5.
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DOI：
10.1590/s1415-475738120140167
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发表时间：
2015-03
影响因子：
2.1
通讯作者：
Akkiprik M
中科院分区：
文献类型：
--
作者：
Güllü G;Peker I;Haholu A;Eren F;Küçükodaci Z;Güleç B;Baloglu H;Erzik C;Özer A;Akkiprik M
The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.
