Molecular evolution of the human enteroviruses: Correlation of serotype with VP1 sequence and application to picornavirus classification
Molecular evolution of the human enteroviruses: Correlation of serotype with VP1 sequence and application to picornavirus classification
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DOI：
10.1128/jvi.73.3.1941-1948.1999
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发表时间：
1999-03-01
影响因子：
5.4
通讯作者：
Pallansch, MA
中科院分区：
文献类型：
--
作者：
Oberste, MS;Maher, K;Pallansch, MA
通过血清中和已鉴定出66种人类肠道病毒血清型，但血清型的分子决定因素尚不清楚。由于小核糖核酸病毒VP1蛋白含有许多中和结构域，我们假设VP1序列应与中和（血清型）相对应，因此与系统发生谱系相对应。为了验证这一假设并分析人类肠道病毒之间的系统发育关系，我们确定了47个人类肠道病毒血清型和10个抗原变异体的原型株的完整VP1序列。我们的序列，连同那些可从GenBank中获得的，包括一个数据库的完整的VP1序列的所有66人肠道病毒血清型加上额外的菌株的7个血清型。从完整的VP1序列构建的系统发育树产生了相同的四个主要集群的基础上部分VP2序列发表的树，相反，VP2树，但是，在VP1树菌株相同的血清型总是单系。在完整的VP1序列的成对比较中，相同血清型的肠道病毒明显区别于异源血清型的肠道病毒，并且型内分歧的界限似乎是约25%的核苷酸序列差异或12%的氨基酸序列差异。两两比较表明，科萨基A11和A15病毒应归类为相同血清型的毒株，科萨基A13和A18病毒也应如此。成对同一性评分也区分了不同簇的肠道病毒和来自不同属的小核糖核酸病毒的肠道病毒。这些数据表明，VP1序列的比较可能是有价值的肠道病毒分型和小核糖核酸病毒的分类，通过协助未分类的小核糖核酸病毒属分配。
Sixty-six human enterovirus serotypes have been identified by serum neutralization, but the molecular determinants of the serotypes are unknown. Since the picornavirus VP1 protein contains a number of neutralization domains, we hypothesized that the VP1 sequence should correspond with neutralization (serotype) and, hence, with phylogenetic lineage. To test this hypothesis and to analyze the phylogenetic relationships among the human enteroviruses, we determined the complete VP1 sequences of the prototype strains of 47 human enterovirus serotypes and 10 antigenic variants. Our sequences, together with those available from GenBank, comprise a database of complete VP1 sequences for all 66 human enterovirus serotypes plus additional strains of seven serotypes. Phylogenetic trees constructed from complete VP1 sequences produced the same four major clusters as published trees based on partial VP2 sequences; in contrast to the VP2 trees, however, in the VP1 trees strains of the same serotype were always monophyletic. In pairwise comparisons of complete VP1 sequences, enteroviruses of the same serotype were clearly distinguished from those of heterologous serotypes, and the limits of intraserotypic divergence appeared to be about 25% nucleotide sequence difference or 12% amino acid sequence difference. Pairwise comparisons suggested that coxsackie A11 and A15 viruses should be classified as strains of the same serotype, as should coxsackie A13 and A18 viruses. Pairwise identity scores also distinguished between enteroviruses of different clusters and enteroviruses from picornaviruses of different genera. The data suggest that VP1 sequence comparisons may be valuable in enterovirus typing and in picornavirus taxonomy by assisting in the genus assignment of unclassified picornaviruses.