Flower extract of Panax notoginseng attenuates lipopolysaccharide-induced inflammatory response via blocking of NF-κB signaling pathway in murine macrophages
Flower extract of Panax notoginseng attenuates lipopolysaccharide-induced inflammatory response via blocking of NF-κB signaling pathway in murine macrophages
复制标题
DOI：
10.1016/j.jep.2008.12.024
复制
发表时间：
2009-03-18
影响因子：
5.4
通讯作者：
Park, Yong-Ki
中科院分区：
文献类型：
--
作者：
Jung, Hyo-Won;Seo, Un-Kyo;Park, Yong-Ki
研究目的：三七（PN）的根通常用于治疗慢性肝病，其治疗能力可以阻止循环中的出血，而PN花（PN-F）在炎症的生物活性及其作用机制方面基本上是未知的。在这项研究中，PN-F甲醇提取物对炎症的药理学作用进行了研究，以解决潜在的治疗或毒性作用在LPS刺激的小鼠巨噬细胞，RAW264.7细胞。材料和方法：产生的NO，PGE（2）和促炎细胞因子（TNF-α和IL-1 β）在上清液中。结果：PN-F提取物能显著抑制LPS刺激的RAW264.7细胞产生NO、PGE 2、TNF-α和IL-1 β，并能显著抑制LPS刺激的RAW264.7细胞产生NO、PGE 2、TNF-α和IL-1 β。此外，PN-F提取物抑制LPS刺激的RAW 264. 7细胞中iNOS、考克斯-2、TNF-α和IL-1 β的mRNA和蛋白表达。PN-F提取物介导RAW264.7细胞减毒的分子机制与抑制ERK 1/2、JNK和p38 MAPK等MAPK分子的磷酸化以及NF-κ B B p65亚基的核转位密切相关。这些结果表明PN-F提取物通过阻断巨噬细胞中的NF-κ B信号传导途径来抑制LPS诱导的炎症反应，并证明PN-F提取物在体外具有抗炎性质。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Aim of the study: The root of Panax notoginseng (PN) is commonly used to treat chronic liver disease with its therapeutic abilities to stop haemorrhage in the circulation, while the PN flower (PN-F) is largely unknown in the biological activities on inflammation and mechanisms of its actions. In this study, the pharmacologic effects of PN-F methanol extract on inflammation were investigated to address potential therapeutic or toxic effects in LPS-stimulated mouse macrophage cells, RAW264.7 cells.Materials and methods: Production of NO, PGE(2) and pro-inflammatory cytokines (TNF-alpha and IL-1 beta) in supernatant. the expression of iNOS, COX-2 and cytokines, the phosphorylation of MAPK moleduces (ERK1/2, JNK and p38 MAPK), and the activation of NF-kappa B in PN-F extract were assayed in LPS-stimulated RAW264.7 cells.Results: PN-F extract significantly inhibited the productions of NO, PGE2, TNF-alpha and IL-1 beta on the LPS-stimulated RAW264.7 cells. In addition, PN-F extract suppressed the mRNA and protein expressions of iNOS, COX-2, TNF-alpha and IL-1 beta in LPS-stimulated RAW264.7 cells. The molecular mechanism of PN-F extract-mediated attenuation in RAW264.7 cells has close a relationship to suppressing the phosphorylation of MAPK molecules such as ERK1/2,JNK and p38 MAPK, and the translocation of NF-kappa B p65 subunit into nuclear.Conclusion: These results indicate that PN-F extract inhibits LPS-induced inflammatory response via the blocking of NF-KB signaling pathway in macrophages, and demonstrated that PN-F extract possesses anti-inflammatory properties in vitro. (C) 2008 Elsevier Ireland Ltd. All rights reserved.