An avian cell line designed for production of highly attenuated viruses
An avian cell line designed for production of highly attenuated viruses
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DOI：
10.1016/j.vaccine.2008.11.066
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发表时间：
2009-01-29
期刊：
影响因子：
5.5
通讯作者：
Sandig, Volker
中科院分区：
文献类型：
--
作者：
Jordan, Ingo;Vos, Ad;Sandig, Volker
几种病毒疫苗，包括非常有前途的载体，如改良的安卡拉牛痘（MVA），在鸡胚成纤维细胞上生产。对原代细胞的依赖使生产复杂化，特别是在大型疫苗接种计划中。对于原代细胞，也不可能产生适于在禽类宿主中增殖的复制缺陷型载体的包装系。为了满足对原代细胞的需求，我们从番鸭的特定组织中获得了永久性细胞系（AGE 1、CR、CS和CA），并对其进行了进一步的修饰：我们证明了来自人腺病毒的结构基因pIX的稳定表达增加了禽类细胞中无关痘病毒的滴度。这种增强似乎是通过诱导热休克介导的，因此提供了一种新的细胞基质，可以进一步减毒疫苗株。(C)2008爱思唯尔有限公司版权所有。
Several viral vaccines, including highly promising vectors such as modified vaccinia Ankara (MVA), are produced on chicken embryo fibroblasts. Dependence on primary cells complicates production especially in large vaccination programs. With primary cells it is also not possible to create packaging lines for replication-deficient vectors that are adapted to proliferation in an avian host. To obviate requirement for primary cells permanent lines from specific tissues of muscovy duck were derived (AGE1.CR, CS, and CA) and further modified: we demonstrate that stable expression of the structural gene pIX from human adenovirus increases titers for unrelated poxvirus in the avian cells. This augmentation appears to be mediated via induction of heat shock and thus provides a novel cellular substrate that may allow further attenuation of vaccine strains. (C) 2008 Elsevier Ltd. All rights reserved.