Transepithelial Transport of α-Lipoic Acid across Human Intestinal Caco-2 Cell Monolayers
Transepithelial Transport of α-Lipoic Acid across Human Intestinal Caco-2 Cell Monolayers
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DOI：
10.1021/jf063624i
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发表时间：
2007-05
影响因子：
6.1
通讯作者：
Naoki Takaishi;Kazutaka Yoshida;H. Satsu;M. Shimizu
中科院分区：
文献类型：
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作者：
Naoki Takaishi;Kazutaka Yoshida;H. Satsu;M. Shimizu
α-硫辛酸（LA）用于具有抗氧化功能的膳食补充剂或食品中。本研究利用人肠道Caco-2细胞单层膜，探讨了α-硫辛酸的肠道吸收机制。LA通过Caco-2细胞单层快速运输，这种运输是能量依赖性的，表明转运蛋白介导的运输机制是相关的。LA输运与pH值密切相关，在酸性pH范围内输运加速。此外，苯甲酸和中链脂肪酸等单羧酸显著抑制LA的转运，表明质子连接的单羧酸转运体（MCT）参与了LA的肠道转运。在运输过程中，LA向抗氧化性更强的二氢硫辛酸的转化也很明显。关键词:Caco-2;α-酸;dihydrolipoic酸;单羧酸转运体；肠道吸收;transepithelial运输
α-Lipoic acid (LA) is used in dietary supplements or food with antioxidative functions. The mechanism for the intestinal absorption of α-lipoic acid was investigated in this study by using human intestinal Caco-2 cell monolayers. LA was rapidly transported across the Caco-2 cell monolayers, this transport being energy-dependent, suggesting transporter-mediated transport to be the mechanism involved. The LA transport was strongly dependent on the pH value, being accelerated in the acidic pH range. Furthermore, such monocarboxylic acids as benzoic acid and medium-chain fatty acids significantly inhibited LA transport, suggesting that a proton-linked monocarboxylic acid transporter (MCT) was involved in the intestinal transport of LA. The conversion of LA to the more antioxidative dihydrolipoic acid was also apparent during the transport process. Keywords: Caco-2; α-lipoic acid; dihydrolipoic acid; monocarboxylic acid transporter; intestinal absorption; transepithelial transport