A strategy for interfering with the formation of thick cell walls in Haematococcus pluvialis by down-regulating the mannan synthesis pathway.
A strategy for interfering with the formation of thick cell walls in Haematococcus pluvialis by down-regulating the mannan synthesis pathway.
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DOI：
10.2139/ssrn.4156114
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发表时间：
2022-08
影响因子：
11.4
通讯作者：
R. Xu;Litao Zhang;WEN-JIE Yu;Jianguo Liu
中科院分区：
文献类型：
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作者：
R. Xu;Litao Zhang;WEN-JIE Yu;Jianguo Liu
与有效的细胞壁破坏相关的挑战仍然是一个重要的瓶颈，限制了从雨生红球藻中提取虾青素的努力。本研究利用现有的转录组学数据和根癌农杆菌介导的转化系统，建立了一个H.雨生藻（pluvialis）菌株，其中下调关键的细胞壁形成相关酶α-1，6-甘露糖基转移酶（HpOCH 1），以努力使细胞壁变薄，从而简化虾青素提取过程。在这些HpOch 1敲低的H. pluvialis细胞导致甘露聚糖组织和已建立的细胞壁的保护能力的显著降低。HpOch 1-组细胞碎裂率较对照组增加58%。至关重要的是，在HpOch 1敲低细胞中，虾青素的合成没有改变。总的来说，这项研究突出了一种新的技术方法，人工细胞壁变薄，提供了一个基础，为进一步努力更有效地利用虾青素资源的H。雨生植物。
The challenges associated with effective cell wall disruption remain an important bottleneck that has restricted efforts to extract astaxanthin from Haematococcus pluvialis. Here, available transcriptomic data and an Agrobacterium tumefaciens-mediated transformation system were used to establish an H. pluvialis strain in which the key cell wall formation-related enzyme α-1,6-mannosyltransferase (HpOCH1) was downregulated in an effort to thin cell walls and thereby simplify the astaxanthin extraction process. The cell wall remodeling activity observed in these HpOch1 knockdown H. pluvialis cells resulted in dramatic reductions in the mannan organization and protective ability of the established cell walls. The cell fragmentation rate increased by 58% in HpOch1- group relative to the control group. Critically, astaxanthin synthesis was not altered in the HpOch1 knockdown cells. Overall, this study highlights a novel technical approach to artificial cell wall thinning, offering a foundation for further efforts to more effectively leverage the astaxanthin resources of H. pluvialis.