Zonulin Regulates Intestinal Permeability and Facilitates Enteric Bacteria Permeation in Coronary Artery Disease.
Zonulin Regulates Intestinal Permeability and Facilitates Enteric Bacteria Permeation in Coronary Artery Disease.
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Zonulin 在冠状动脉疾病中调节肠道渗透性并促进肠道细菌渗透。
DOI：
10.1038/srep29142
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发表时间：
2016-06-29
影响因子：
4.6
通讯作者：
Zeng C
中科院分区：
文献类型：
--
作者：
Li C;Gao M;Zhang W;Chen C;Zhou F;Hu Z;Zeng C
一些研究报道了肠道细菌与动脉粥样硬化之间的联系。在动脉粥样硬化斑块中检测到肠杆菌科细菌16S核糖体RNA （rRNA）基因。肠道细菌如何进入血液尚不清楚。Zonulin可逆调节肠通透性（IP），糖尿病、肥胖症患者循环Zonulin水平升高，均为动脉粥样硬化的危险因素。目前尚不清楚循环zonulin水平是否在冠状动脉疾病（CAD）患者中发生改变并调节IP。采用454焦磷酸测序法检测血样中细菌的16S rRNA基因。采用酶联免疫吸附法（ELISA）检测zonulin水平。共聚焦免疫荧光显微镜检测zonulin的分布。透射电镜观察细菌和Caco-2细胞表面微观结构。在CAD患者的样本中检测到细菌16S rRNA基因的高度多样性，其中大多数（99.4%）属于肠杆菌科。Rahnella。冠心病患者血浆zonulin水平明显升高。荧光假单胞菌暴露显著增加zonulin的表达，并以时间依赖性方式降低IP。升高的zonulin增加了IP，并可能通过破坏紧密连接促进肠道易位，这可能解释了血液样本中观察到的细菌16S rRNA基因的高度多样性。
Several studies have reported an association between enteric bacteria and atherosclerosis. Bacterial 16S ribosomal RNA (rRNA) gene belong to Enterobacteriaceae have been detected in atherosclerotic plaques. How intestinal bacteria go into blood is not known. Zonulin reversibly modulate intestinal permeability (IP), the circulating zonulin levels were increased in diabetes, obesity, all of which are risk factors for atherosclerosis. It is unclear whether the circulating zonulin levels were changed in coronary artery disease (CAD) patients and modulate IP. The 16S rRNA gene of bacteria in blood sample was checked by 454 pyrosequencing. The zonulin levels were determined by enzyme-linked immunosorbent assay (ELISA) methods. The distribution of zonulin was detected by confocal immunofluorescence microscopy. Bacteria and Caco-2 cell surface micro-structure were checked by transmission electron microscopy. A high diversity of bacterial 16S rRNA gene can be detected in samples from CAD patients, most of them (99.4%) belong to Enterobacteriaceaes, eg. Rahnella. The plasma zonulin levels were significantly higher in CAD patients. Pseudomonas fluorescens exposure significantly increased zonulin expression and decreased IP in a time dependent manner. The elevated zonulin increase IP and may facilitate enteric translocation by disassembling the tight junctions, which might explain the observed high diversity of bacterial 16S rRNA genes in blood samples.