High speed separation of DNA fragments by capillary electrophoresis in poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock polymer
High speed separation of DNA fragments by capillary electrophoresis in poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock polymer
复制标题
DOI：
10.1002/elps.1150191416
复制
发表时间：
1998-10-01
期刊：
影响因子：
2.9
通讯作者：
Chu, B
中科院分区：
文献类型：
--
作者：
Liang, DH;Chu, B
本文研究了用25%w/v的三嵌段共聚物F127（PEO_（99）PPO_（69）PEO_（99），其中PEO和PPO分别表示聚环氧乙烷和聚环氧丙烷）快速分离DNA片段。提出了缩短毛细管有效长度和增大电场强度两种缩短运行时间的方法。在短毛细管中，分离介质的筛分能力与初始带宽以及作为行进距离的函数的带宽扩展主导分辨率;在高电场强度下，焦耳加热可能使分离恶化。通过考虑这两种效应，Phi X174/HaeIII消化物可以在100秒内通过使用8 mm有效长度、50 μ m直径的毛细管在300 V/cm下操作而分离。
The high speed separation of DNA fragments by using a triblock copolpmer, 25% w/v F127 (PEO99PPO69PEO99 with PEO and PPO denoting polyethylene oxide and polypropylene oxide, respectively) which is easy to handle and does not need coating of the quartz capillary, has been investigated. Two ways to decrease the run time are presented: one is to shorten the effective capillary length and the other to increase the electric field strength. In a short capillary, the sieving ability of the separation medium versus the initial band width, and the band width spreading as a function of distance traveled dominate the resolution; at high electric field strength, Joule heating could deteriorate the separation. By taking both effects into account, the Phi X174/HaeIII digest could be separated within 100s by using an 8 mm effective length, 50 mu m diameter capillary operating at 300 V/cm.