Telomere length and telomerase in a well-characterized sample of individuals with major depressive disorder compared to controls.
Telomere length and telomerase in a well-characterized sample of individuals with major depressive disorder compared to controls.
复制标题
与对照组相比，重度抑郁症个体的明确特征样本中的端粒长度和端粒酶。
DOI：
10.1016/j.psyneuen.2015.04.004
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发表时间：
2015
影响因子：
3.7
通讯作者：
Wong,Kw
中科院分区：
文献类型：
--
作者：
Simon,NaomiM;Walton,ZandraE;Bui,Eric;Prescott,Jennifer;Hoge,Elizabeth;Keshaviah,Aparna;Schwarz,Noah;Dryman,Taylor;Ojserkis,RebeccaA;Kovachy,Benjamin;Mischoulon,David;Worthington,John;DeVivo,Immaculata;Fava,Maurizio;Wong,Kw
背景白细胞端粒长度（LTL）是细胞更新和氧化应激的标志。研究表明，重度抑郁症（MDD）与氧化应激有关，但MDD和LTL的检查产生了混合的结果，可能是因为测量方法和未测量的混杂因素的差异。本研究探讨LTL和端粒酶活性在166个人与MDD相比，166个年龄和性别匹配的对照组的任何精神疾病，使用经过验证的检测和临床评估方法，并控制一系列潜在的confusers.MethodsSubjects年龄在18至70岁进行了评估，由训练有素的评分员和血液LTL和端粒酶活性测量。使用Southern印迹分析LTL并用qPCR复制，结果MDD患者端粒长度无显著性差异[平均值（SD）= 9.1通过Southern印迹（p= 0.65）或通过确证性qPCR（p= 0.91）测定，与对照组[平均值（SD）= 8.9（2.5）kbp]相比，控制潜在混杂因素不会改变结果。总体而言，MDD诊断的端粒酶活性无差异（p= 0.40），但即使在控制了潜在的混杂因素后，MDD的影响也因性别而显著改变（t（299）= 2.67，p = 0.0079），仅MDD男性患者的端粒酶活性显著高于对照组。在具有临床意义的慢性MDD患者和匹配的对照组中，同时评估端粒长度和端粒酶活性的有效检查未能提供MDD与较短LTL相关的强有力证据，而MDD组端粒酶活性较低。
BackgroundLeukocyte telomere length (LTL) is a marker of cellular turnover and oxidative stress. Studies suggest major depressive disorder (MDD) is associated with oxidative stress, but examinations of MDD and LTL have yielded mixed results, likely because of differences in measurement methods and unmeasured confounding. This study examined LTL and telomerase activity in 166 individuals with MDD compared to 166 age- and gender-matched matched controls free of any psychiatric disorder, using well-validated assays and clinical assessment methods, and controlling for a range of potential confounders.MethodsSubjects aged 18 to 70 were evaluated by trained raters and provided blood for LTL and telomerase activity measurement. LTL was assayed using Southern blot and replicated with qPCR, and telomerase activity was assayed with a repeat amplification protocol using a commercial kit.ResultsThere was no significant difference in telomere length for individuals with MDD [mean (SD) = 9.1 (3.0) kbp] compared to controls [mean(SD) = 8.9(2.5) kbp] measured by Southern blot (p= 0.65) or by confirmatory qPCR (p= 0.91) assays. Controlling for potential confounders did not alter the results. Telomerase activity did not differ by MDD diagnosis overall (p= 0.40), but the effect of MDD was significantly modified by gender (t(299) = 2.67,p= 0.0079) even after controlling for potential confounders, with telomerase activity significantly greater only in males with MDD versus controls.ConclusionOur well-characterized, well-powered examination of concurrently assessed telomere length and telomerase activity in individuals with clinically significant, chronic MDD and matched controls failed to provide strong evidence of an association of MDD with shorter LTL, while telomerase activity was lower in men with MDD.