Involvement of programmed death-ligand 2 (PD-L2) in the development of experimental allergic conjunctivitis in mice
Involvement of programmed death-ligand 2 (PD-L2) in the development of experimental allergic conjunctivitis in mice
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DOI：
10.1136/bjo.2006.091314
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发表时间：
2006-08-01
影响因子：
4.1
通讯作者：
Ueno, H.
中科院分区：
文献类型：
--
作者：
Fukushima, A.;Yamaguchi, T.;Ueno, H.
背景/目的：程序性死亡-1（PD-1）及其配体参与实验性过敏性气道疾病。在这里，作者的目的是研究PD-1及其配体是否参与实验性过敏性结膜炎（EC）在mice.Methods：EC的发展在Balb/c小鼠诱导的主动免疫与短豚草花粉（RW）在明矾。10天后（第10天），用含有RW的滴眼剂攻击小鼠。攻击后24小时，收获结膜、脾和血清用于组织学分析、细胞因子测定和RW特异性IG水平的测量。主动免疫的小鼠进行了治疗与抗PD-1，抗PD-L1，抗PD-L2抗体（抗体），或正常大鼠免疫球蛋白G（nrIgG）在诱导（第0，2，4，6，和8天）或效应（2小时前RW挑战第10天）phase.Results：抗体治疗在诱导阶段并没有影响嗜酸性粒细胞浸润，但免疫反应被调制。与此相反，治疗抗PD-L2抗体，但不抗PD-1或抗PD-L1抗体，在效应阶段显着增加嗜酸性粒细胞浸润到结膜，而不影响全身免疫responses.Conclusions：类似过敏性气道炎症，PD-L2参与EC的发展在效应阶段，但不是诱导阶段。
Background/aim: Involvement of programmed death-1 (PD-1) and its ligands has been demonstrated in experimental allergic airway disease. Here, the authors aimed to examine whether PD-1 and its ligands are involved in the development of experimental allergic conjunctivitis (EC) in mice.Methods: EC was induced in Balb/c mice by active immunisation with short ragweed pollen (RW) in alum. 10 days later (day 10), the mice were challenged with eye drops containing RW. 24 hours after the challenge, conjunctivas, spleens, and sera were harvested for histological analysis, cytokine assays, and measurement of RW specific Ig levels. The actively immunised mice were treated with anti-PD-1, anti-PD-L1, anti-PD-L2 antibodies (Abs), or normal rat immunoglobulin G (nrIgG) during either the induction (day 0, 2, 4, 6, and 8) or the effector (2 hours before RW challenge on day 10) phase.Results: Ab treatment during the induction phase did not affect eosinophil infiltration although immune responses were modulated. In contrast, treatment with anti-PD-L2 Ab, but not anti-PD-1 or anti-PD-L1 Ab, during the effector phase significantly increased eosinophil infiltration into the conjunctiva without affecting systemic immune responses.Conclusions: Similar to allergic airway inflammation, PD-L2 is involved in the development of EC during the effector phase but not the induction phase.