Gateway to the Golgi: molecular mechanisms of nucleotide sugar transporters
Gateway to the Golgi: molecular mechanisms of nucleotide sugar transporters
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DOI：
10.1016/j.sbi.2019.03.019
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发表时间：
2019-08-01
影响因子：
6.8
通讯作者：
Newstead, Simon
中科院分区：
文献类型：
--
作者：
Parker, Joanne L.;Newstead, Simon
高尔基体作为翻译后修饰、蛋白质分选和质量控制的枢纽，在分泌途径中起着核心作用。到目前为止，有很少的结构或生化信息有关的功能，运输，居住在这个细胞器。核苷酸糖转运蛋白的SLC 35家族将细胞质中活化的糖分子和硫酸盐的合成与管腔转移酶连接，所述管腔转移酶在糖基化和硫酸化期间催化它们与蛋白质和脂质的附着。最近的GDP-甘露糖转运蛋白的晶体结构揭示了直接配体识别和转运的关键序列基序。进一步的生化研究意外地发现激活转运蛋白需要短链脂质，这表明了高尔基体内转运调节的可能途径。
The Golgi apparatus plays a central role in the secretory pathway as a hub for posttranslational modification, protein sorting and quality control. To date, there is little structural or biochemical information concerning the function of transporters that reside within this organelle. The SLC35 family of nucleotide sugar transporters link the synthesis of activated sugar molecules and sulfate in the cytoplasm, with the luminal transferases that catalyse their attachment to proteins and lipids during glycosylation and sulfation. A recent crystal structure of the GDP-mannose transporter has revealed key sequence motifs that direct ligand recognition and transport. Further biochemical studies unexpectedly found a requirement for short chain lipids in activating the transporter, suggesting a possible route for transport regulation within the Golgi.