Anticonvulsant effect of Uncaria rhynchophylla (Miq) Jack. in rats with kainic acid-induced epileptic seizure
Anticonvulsant effect of Uncaria rhynchophylla (Miq) Jack. in rats with kainic acid-induced epileptic seizure
复制标题
DOI：
10.1142/s0192415x9900029x
复制
发表时间：
1999-01-01
影响因子：
5.7
通讯作者：
Lin, JG
中科院分区：
文献类型：
--
作者：
Hsieh, CL;Chen, MF;Lin, JG
本研究探讨钩藤的抗惊厥作用及其作用的生理机制。实验选用雄性SD大鼠70只。将34只大鼠分为5组：1)对照组(n=6)：腹腔注射。2)UR1000组(n=10)，3)UR500组(n=6)，4)UR250组，分别腹腔注射URR1000、500、250 mg/kg。5)对照组：卡马西平20 mg/kg，ip。KA给药前30分钟。用药前15min至KA用药后3h监测动物的行为和脑电。在整个实验过程中，每隔10分钟计算一次湿狗抖动的次数。其余36只大鼠于给予KA后1h测定大脑皮层过氧化脂质水平。将大鼠分为6组：1)正常组：不给予任何治疗；2)对照组：腹腔注射KA(12 mg/kg)，3)UR1000组，4)UR500组，5)UR250组，分别给予URR1000、500、250 mg/kg。6)对照组：卡马西平20 mg/kg，ip。KA给药前30分钟。结果表明，UR 1000 mg/kg和500 mg/kg均可降低KA所致犬湿抖的发生率，UR 250 mg/kg组和卡马西平20 mg/kg组未见类似作用。UR 1000 mg/kg、500 mg/kg、250 mg/kg和卡马西平20 mg/kg可降低KA诱导的大脑皮层脂质过氧化水平，并呈剂量依赖关系。提示UR的抗惊厥作用可能与其抑制脑内脂质过氧化作用有关。
This study investigated the anticonvulsant effect of Uncaria rhynchophylla (UR) and the physiological mechanisms of its action in rats. A total of 70 male Sprague-Dawley (SD) rats were selected for study. Thirty four of these rats were divided into 5 groups as follows: 1) Control group (n = 6): received intraperitoneal injection (i.p.) of kainic acid (KA, 12 mg/kg); 2) UR1000 group (n = 10), 3) UR500 group (n = 6) 4) UR250 group, received UR 1000, 500, 250 mg/kg i.p. 30 min prior to KA administration, respectively; 5) Contrast group: received carbamazepine 20 mg/kg i.p. 30 min prior to KA administration. Behavior and EEG were monitored from 15 min prior to drug administration to 3 hours after KA administration. The number of wet dog shakes were counted at 10 min intervals throughout the experimental course. The remaining 36 rats were used to measure the lipid peroxide level in the cerebral cortex one hour after KA administration. These rats were divided into 6 groups of B rats as follows: 1) Normal group: no treatment was given; 2) Control group: received KA (12 mg/kg) i.p.; 3) UR1000 group, 4) UR500 group, 5) UR250 group, received UR 1000, 500, 250 mg/kg i.p. 30 min prior to KA administration, respectively; 6) Contrast group: received carbamazepine 20 mg/kg i.p. 30 min prior to KA administration. Our results indicated that both UR 1000 and 500 mg/kg decreased the incidence of KA-induced wet dog shakes, no similar effect was observed in the UR 250 mg/kg and carbamazepine 20 mg/kg group. Treatment with UR 1000 mg/kg, 500 mg/kg, or 250 mg/kg and carbamazepine 20 mg/kg decreased KA-induced lipid peroxide level in the cerebral cortex and was dose-dependent. These findings suggest that the anticonvulsant effect of UR possibly results from its suppressive effect on lipid peroxidation in the brain.