Discrimination of solvent from protein regions in native Fouriers as a means of evaluating heavy-atom solutions in the MIR and MAD methods
Discrimination of solvent from protein regions in native Fouriers as a means of evaluating heavy-atom solutions in the MIR and MAD methods
复制标题
DOI：
10.1107/s0907444998012657
复制
发表时间：
1999-02-01
期刊：
影响因子：
--
通讯作者：
Berendzen, J
中科院分区：
文献类型：
--
作者：
Terwilliger, TC;Berendzen, J
本机傅立叶的自动化检查测试作为一种手段的MAD和MIR方法用于大分子晶体学的重原子溶液的评价。结果发现，在电子密度图的高，低密度变化的不同区域的存在是一个很好的指标的MIR和MAD方法中的重原子解决方案的正确性。该方法可用于评估重原子的解决方案，在MAD和MIR结构的解决方案，并确定手性的结构，如果异常数据已被测量。
An automated examination of the native Fourier is tested as a means of evaluation of a heavy-atom solution in MAD and MIR methods for macromolecular crystallography. It is found that the presence of distinct regions of high and low density variation in electron-density maps is a good indicator of the correctness of a heavy-atom solution in the MIR and MAD methods. The method can be used to evaluate heavy-atom solutions during MAD and MIR structure solutions and to determine the handedness of the structure if anomalous data have been measured.