Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan.
Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan.
复制标题
DOI：
10.1292/jvms.14-0624
复制
发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Norimine J
中科院分区：
文献类型：
--
作者：
Mekata H;Sekiguchi S;Konnai S;Kirino Y;Horii Y;Norimine J
水平传播被认为是牛白血病病毒（BLV）的主要感染途径，高病毒载量的牛被认为是牛群中的主要传染源。然而，病毒载量和感染风险之间的相关性不足以作为BLV控制策略的基础。本文对某地区BLV感染的流行病学和传染源进行了调查。2013-2014年，在日本宫崎两个相邻地区的117个农场的1，823头牛中调查了BLV感染。在14个农场的88头牛中检测到BLV血清阳性样品。系统发育分析表明，94%的菌株聚成基因型I和基因型III的其余隔离。在基因型I中，遗传上不同的菌株在每个农场传播，感染少于3个拷贝/100个细胞的牛在30个月内不会将BLV传播给其他牛。这是首次报道现场条件下病毒载量与病毒水平传播相关的具体数据。这些数据有助于农民和兽医了解BLV感染牛的状况。该研究有助于BLV感染控制和有效根除BLV计划的发展。
Horizontal transmission is recognized as a major infection route for bovine leukemia virus (BLV), and cattle with high viral loads are considered to be a major infectious source in a herd. However, a correlation between viral loads and the risk of infection has been insufficient to use as a foundation for BLV control strategies. In this report, we examined the epidemiology of BLV infection and the infectious source in a local area. In 2013–2014, BLV infection was investigated in 1,823 cattle from 117 farms in two adjacent districts, Miyazaki, Japan. Seropositive samples for BLV were detected with 88 cattle and in 14 farms. Phylogenetic analysis revealed that 94% of the isolates clustered into genotype I and the remaining isolate into genotype III. Among genotype I, genetically distinct strains were spread at each farm, and cattle infected with less than 3 copies/100 cells did not transmit BLV to other cattle for more than thirty months. This is the first report of concrete data of viral load in relation to viral horizontal transmission under the field condition. The data facilitate farmers and veterinarians understanding the status of BLV infected cattle. This research contributes to BLV infection control and the development of effective BLV eradication programs.