Distribution and hormone regulation of estrogen receptor immunoreactive cells in the hippocampus of male and female rats
Distribution and hormone regulation of estrogen receptor immunoreactive cells in the hippocampus of male and female rats
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DOI：
10.1002/(sici)1096-9861(19971201)388:4
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发表时间：
1997-12
影响因子：
2.5
通讯作者：
N. Weiland;C. Orikasa;S. Hayashi;B. McEwen
中科院分区：
文献类型：
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作者：
N. Weiland;C. Orikasa;S. Hayashi;B. McEwen
雌激素调节海马的突触可塑性和生理学以及由海马介导的学习行为。雌激素处理改变了海马CA 1区树突棘和突触的密度、N-甲基-D-天冬氨酸（NMDA）结合位点的数量、NMDA受体亚基NR 1蛋白的水平、蝇蕈醇与γ-氨基丁酸（GABA）A受体的结合以及谷氨酸脱羧酶信息的水平。此外，这些参数中的一些在对雌激素治疗的反应中表现出性别差异。为了确定雌激素对海马的直接影响，并确定雌激素反应性的性别差异是否是由于雌激素受体（ER）水平的性别差异，我们使用免疫细胞化学与AS 409抗体来绘制雄性和雌性大鼠海马中ER免疫反应（ER-ir）细胞的位置。我们发现：（1）ER似乎位于中间神经元，而不是锥体细胞或颗粒细胞神经元;（2）ER-ir细胞最集中地位于齿状回门和CA 1区的放射层;（3）ER-ir细胞密度在齿状回门和CA 1区呈吻尾梯度，（4）海马中ER-ir细胞的数量或免疫染色强度均无性别差异，（5）雌激素下调雄性和雌性大鼠的ER水平，（6）海马中ER-ir细胞的平均染色强度约为下丘脑ER-ir细胞的25%。由此，我们可以得出结论，雌激素可以对海马神经元产生直接影响，并且雌激素反应性的任何性别差异都是由于海马中ER水平或功能的性别差异以外的原因。J. Comp.神经元388：603-612，1997.© 1997 Wiley利斯公司
Estrogen regulates the synaptic plasticity and physiology of the hippocampus as well as learning behaviors that are mediated by the hippocampus. The density of dendritic spines and synapses, the number of N‐methyl‐D‐aspartate (NMDA) binding sites, the levels of NMDA receptor subunit NR1 protein, muscimol binding to the γ‐amino butyric acid (GABA)A receptor, and levels of glutamic acid decarboxylase message in the CA1 region of the hippocampus are altered with estrogen treatment. In addition, some of these parameters exhibit sex differences in their response to estrogen treatment. To establish that estrogen can have a direct effect on the hippocampus and to determine whether or not sex differences in estrogen responsiveness are due to sex differences in estrogen receptor (ER) levels, we used immunocytochemistry with the AS409 antibody to map the location of ER‐immunoreactive (ER‐ir) cells in the hippocampus of male and female rats. We found that (1) the ERs appear to be in interneurons rather than pyramidal or granule cell neurons, (2) ER‐ir cells are located in greatest concentration in the hilus of the dentate gyrus and the stratum radiatum of the CA1 region, (3) the density of ER‐ir cells exhibits a rostral to caudal gradient in the hilus and the CA1 regions, (4) there are no sex differences in either the number or immunostaining intensity of ER‐ir cells in the hippocampus, (5) the ER levels are down‐regulated by estrogen in both male and female rats, and (6) the mean intensity of staining for the ER‐ir cells in the hippocampus is about 25% of that in the ER‐ir cells of the hypothalamus. From this, we can conclude that estrogen can have a direct effect on hippocampal neurons and that any sex differences in estrogen responsiveness is due to something other than sex differences in ER levels or function in the hippocampus. J. Comp. Neurol. 388:603–612, 1997. © 1997 Wiley‐Liss, Inc.