Rhamnan sulfate extracted from Monostroma nitidum attenuates blood coagulation and inflammation of vascular endothelial cells
Rhamnan sulfate extracted from Monostroma nitidum attenuates blood coagulation and inflammation of vascular endothelial cells
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DOI：
10.1007/s11418-019-01289-5
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发表时间：
2019-06-01
影响因子：
3.3
通讯作者：
Suzuki, Koji
中科院分区：
文献类型：
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作者：
Okamoto, Takayuki;Akita, Nobuyuki;Suzuki, Koji
硫酸鼠李糖（RS）是一种从硝酸单基质中分离得到的鼠李糖骨架多糖。像肝素一样，它在抗凝血酶存在时发挥抗凝血活性。内皮细胞促进凝血和血管炎症之间的相互作用。在本研究中，我们以人脐静脉内皮细胞为实验对象，比较了RS与肝素在存在或不存在炎症因子的情况下对凝血和血管内皮细胞的影响。我们发现，在抗凝血酶和肝素存在的情况下，RS显著增强对凝血酶和Xa因子的抑制作用，并且RS抑制内皮细胞中组织因子的表达和血管性血血病因子的释放，无论是否使用脂多糖、肿瘤坏死因子-或凝血酶。肝素对内皮细胞炎症没有任何影响。我们的研究结果表明，RS与肝素一样，是一种抗凝血酶依赖性抗凝剂，与肝素不同，RS是一种有效的抗炎剂，作用于血管内皮细胞。
Rhamnan sulfate (RS) is a polysaccharide with a rhamnose backbone isolated from Monostroma nitidum. Like heparin, it exerts anticoagulant activity in the presence of antithrombin. Endothelial cells facilitate the crosstalk between blood coagulation and vascular inflammation. In this study, we compared the effect of RS with that of heparin on blood coagulation and vascular endothelial cells in the presence or absence of inflammatory factors, using human umbilical vein endothelial cells. We found that RS significantly enhances inhibition of thrombin and factor Xa in the presence of antithrombin as well as heparin, and that RS inhibits tissue factor expression and von Willebrand factor release from the endothelial cells treated with or without lipopolysaccharide, tumor necrosis factor-, or thrombin. Heparin did not show any effects on endothelial cell inflammation. Our findings suggest that RS, like heparin, is an antithrombin-dependent anticoagulant and, unlike heparin, is a potent anti-inflammatory agent acting on vascular endothelial cells.