Temperature and photoperiod drive Crassostrea gigas reproductive internal clock
Temperature and photoperiod drive Crassostrea gigas reproductive internal clock
复制标题
DOI：
10.1016/j.aquaculture.2005.02.038
复制
发表时间：
2005-11-14
期刊：
影响因子：
4.5
通讯作者：
Pouvreau, S
中科院分区：
文献类型：
--
作者：
Fabioux, C;Huvet, A;Pouvreau, S
本研究研究了长牡蛎在受控条件下一年多的配子发生周期，重点是配子发生的启动。本工作还分析了温度和光周期在牡蛎繁殖调控中的作用。亲鱼保持在自然(NC)、加速(AC)和常冬(WC)的温光周期条件下，并自由取食。采用生物测量、性比测定、组织学和性腺充盈指数等方法对其生殖方式进行了定性和定量的分析。每一种实验性的处理都导致了不同的增长和资源分配策略。配子发生周期似乎完全受温度/光周期参数的调节、加速或延迟。温度在生殖器有丝分裂的调控中起着关键作用。无论牡蛎的生理状态如何，冬季温度(8-11摄氏度)都会引发和维持牡蛎的增殖。生殖细胞的成熟似乎是温度的函数，可以在较低的温度下进行，而在冬季条件下，8℃就可以获得成熟的牡蛎。在这项研究中使用的三种调节方法，允许全年配子的产生，包括在秋季休眠期。此外，成熟牡蛎的库存可以在几个月内保持低温，以便在水产养殖生产需要时生产Spat。(C)2005年，爱思唯尔出版。
This study examined the gametogenic cycle of Crassostrea gigas in controlled conditions over one year, with a focus on the initiation of gametogenesis. This work analysed also the role of temperature and photoperiod in the regulation of oyster reproduction. Broodstock were maintained in natural (NC), accelerated (AC) and perpetual winter (WC) conditions of temperature and photoperiod, with feeding ad libitum. Qualitative and quantitative analyses of the reproductive pattern were performed using biometric measurement approach, sex ratio determination, histology and a gonad filling index. Each experimental treatment led to different strategies for growth and resource allocation. The gametogenic cycle, appeared entirely modulated, accelerated or delayed, by coupled temperature/photoperiod parameters. Temperature played a key role in gonial mitosis regulation. Gonia proliferation was set off and sustained by winter temperature (8-11 degrees C) whatever the physiological state of oysters. Maturation of germ cells appeared to be a function of temperature and could proceed at low temperature, while ripe oysters were obtained at 8 degrees C in winter conditioning. The three conditioning methods used in this study, allowed the production of gametes throughout the year, including in the autumnal resting period. Moreover, stocks of ripe oysters could be maintained at low temperature during several months to produce spat when desired for aquaculture production. (C) 2005 Published by Elsevier B.V.