A conserved domain of alkaline phosphatase expression in the Malpighian tubules of dipteran insects
A conserved domain of alkaline phosphatase expression in the Malpighian tubules of dipteran insects
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DOI：
10.1242/jeb.01156
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Dow, JAT
中科院分区：
文献类型：
--
作者：
Cabrero, P;Pollock, VP;Dow, JAT
马氏管（肾）是昆虫生殖调节系统的关键组成部分，在数量和长度上表现出相应的巨大差异。最近，果蝇小管的组织已被阐明增强子捕获，和阵列的功能特性已被证明与功能结构域对齐。在果蝇中，有一个较低的小管结构域，这与碱性磷酸酶的表达相一致，并描绘了小管的吸收区域。在这里，这些观察扩展到三个双翅目疾病的载体（埃及伊蚊，斯氏按蚊和Glossina morsitans）和非双翅目外类群，Schistocerca gregaria（直翅目）。尽管在细胞数量和大小的巨大范围内，碱性磷酸酶被发现在顶端表面的下10%的双翅目小管，但在直翅目小管内无处。碱性磷酸酶下小管结构域因此在双翅目中是保守的。在果蝇中，星状细胞在按蚊或伊蚊小管的下小管域中没有发现，证实了该域的独特遗传特性。正如以前报道的那样，我们未能在Schistocerca中找到星状细胞，但值得注意的是，在Glossina中也未能找到它们，Glossina是与果蝇关系最密切的双翅目昆虫。星状细胞是双翅目昆虫所特有的，并且在双翅目昆虫中普遍存在的正统观点可能因此需要修正。
Malpighian (renal) tubules are key components of the insect osmoregulatory system and show correspondingly great diversity in both number and length. Recently, the organisation of the Drosophila melanogaster tubule has been elucidated by enhancer trapping, and an array for functional properties has been shown to align with the functional domains. In Drosophila, there is a lower tubule domain, which coincides with expression of alkaline phosphatase and delineates the absorptive region of the tubule. Here, these observations are extended to three dipteran vectors of disease (Aedes aegypti, Anopheles stephensii and Glossina morsitans) and a non-dipteran outgroup, Schistocerca gregaria (Orthoptera). Despite a huge range in cell number and size, alkaline phosphatase was found on the apical surface of the lower 10% of each of the dipteran tubules but nowhere within the orthopteran tubule. An alkaline phosphatase lower tubule domain is thus conserved among Diptera.Cell counts are also provided for each species. As in Drosophila, stellate cells are not found in the lower tubule domain of Anopheles or Aedes tubules, confirming the unique genetic identity of this domain. As previously reported, we failed to find stellate cells in Schistocerca but, remarkably, also failed to find them in Glossina, the dipteran most closely related to Drosophila. The orthodoxy that stellate cells are unique to, and general among, Diptera may thus require revision.