Chilling-Enhanced Photooxidation : The Peroxidative Destruction of Lipids during Chilling Injury to Photosynthesis and Ultrastructure.
Chilling-Enhanced Photooxidation : The Peroxidative Destruction of Lipids during Chilling Injury to Photosynthesis and Ultrastructure.
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DOI：
10.1104/pp.83.2.272
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发表时间：
1987-02
期刊：
影响因子：
7.4
通讯作者：
Robert R. Wise;Aubrey W. Naylor
中科院分区：
文献类型：
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作者：
Robert R. Wise;Aubrey W. Naylor
以冷敏感黄瓜（Cucumis sativus L.）和抗冷（CR）豌豆（Pisum sativum L.）。光合作用和呼吸作用的速率，测量为O（2）交换，被发现在5至35摄氏度的温度范围内，这两个物种是相当的。在5摄氏度的强光（每秒每平方米1000微爱因斯坦）下冷却12小时，使离体豌豆叶片的CO（2）吸收量减少75%，而黄瓜的CO（2）吸收量在2小时内减少到零。呼吸是不受影响的两个物种的冷冻和光处理。虽然超微结构的改变是明显的，在这两个物种的叶绿体，黄瓜的影响更快，更严重。研究了光氧化脂质过氧化反应的机理。最大乙烷产生发生在CS黄瓜在低温（5摄氏度）和高光（1000微爱因斯坦每平方米每秒）。阿特拉津，光合电子传递的抑制剂，几乎完全停止了这种寒冷和光诱导的乙烷生产。这些数据，与所附文章（RR Wise，AW Naylor 1986 Plant Physiol 83：278-282）中报道的数据一起表明，在冷冻增强的光氧化过程中，黄瓜叶绿体中产生超氧阴离子自由基（可能通过Mehler型反应）。平行试验进行了豌豆，CR物种。如果用除草剂百草酮（一种已知的O（2）（-）产生的效应剂）对豌豆离体叶片进行预处理，它们只能在寒冷和光照下产生乙烷。即便如此，豌豆没有表现出脂质过氧化6小时，在这个时候乙烷的生产开始，并在相同的速度为冷冻和辐照黄瓜叶。结果表明，豌豆有一个内源性的机制（S）的清除有毒的氧物种之前，脂质过氧化。豌豆在寒冷、光照和百草枯存在下6小时后，这种机制就被打破了。
Chilling-induced photooxidation was studied in detached leaves of chilling-sensitive (CS) cucumber (Cucumis sativus L.) and chilling resistant (CR) pea (Pisum sativum L.). The rates of photosynthesis and respiration, measured as O(2) exchange, were found to be comparable in the two species over a temperature range of 5 to 35 degrees C. Chilling at 5 degrees C for 12 hours in high light (1000 microeinsteins per square meter per second) decreased CO(2) uptake 75% in detached pea leaves whereas CO(2) uptake by cucumber was reduced to zero within 2 hours. Respiration was unaffected in either species by the chilling and light treatment. Although ultrastructural alterations were apparent in chloroplasts of both species, cucumber's were affected sooner and more severely. The mechanism of photooxidative lipid peroxidation was investigated by following the production of ethane gas under a variety of conditions. Maximum ethane production occurred in the CS cucumber at low temperature (5 degrees C) and high light (1000 microeinsteins per square meter per second). Atrazine, an inhibitor of photosynthetic electron transport, almost completely halted this chilling- and light-induced ethane production. These data, taken with those reported in an accompanying article (RR Wise, AW Naylor 1986 Plant Physiol 83: 278-282) suggest that the superoxide anion radical is generated in cucumber chloroplasts (probably via a Mehler-type reaction) during chilling-enhanced photooxidation. Parallel experiments were conducted on pea, a CR species. Detached pea leaves could only be made to generate ethane in the cold and light if they were pretreated with the herbicide parquat, a known effector of O(2) (-) production. Even so, pea showed no lipid peroxidation for 6 hours, at which time ethane production began and was at a rate equal to that for the chilled and irradiated cucumber leaves. The results indicate that pea has an endogenous mechanism(s) for the removal of toxic oxygen species prior to lipid peroxidation. This mechanism breaks down in pea after 6 hours in the cold, light, and the presence of paraquat.