THE MOUSE-Y-STAR CHROMOSOME INVOLVES A COMPLEX REARRANGEMENT, INCLUDING INTERSTITIAL POSITIONING OF THE PSEUDOAUTOSOMAL REGION
THE MOUSE-Y-STAR CHROMOSOME INVOLVES A COMPLEX REARRANGEMENT, INCLUDING INTERSTITIAL POSITIONING OF THE PSEUDOAUTOSOMAL REGION
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DOI：
10.1159/000133152
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
WASHBURN, LL
中科院分区：
文献类型：
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作者：
EICHER, EM;HALE, DW;WASHBURN, LL
小鼠Y*染色体的细胞学分析揭示了一个复杂的重排，包括获得一个功能着丝粒和着丝粒异染色质，并将该染色体片段连接到正常Y染色体的远端。这种重排将Y*染色体远端的Y短臂区和假常染色体间区定位在新获得的着丝粒远端的位置。此外，大部分假常染色体区域是倒置的。X和Y*染色体之间的重组产生了两条新的性染色体：(1)一条由X染色体组成的大染色体，它的远端附着在所有Y*染色体上，但缺少着丝粒区域（X(Y)*）；(2)一条小染色体，它包含Y*染色体的着丝粒部分，附着在X染色体的g带阴性物质上（Y*X）。继承X(Y)*染色体的小鼠发育为不育的雄性，而继承Y*X染色体的小鼠发育为可育的雌性。从X(Y)*雄性中恢复相同数量的重组和非重组后代，支持了哺乳动物X和Y染色体之间的重组是初级精母细胞成功完成精子发生和形成功能精子所必需的假设。
Cytological analysis of the mouse Y* chromosome revealed a complex rearrangement involving acquisition of a functional centromere and centromeric heterochromatin and attachment of this chromosomal segment to the distal end of a normal Y chromosome. This rearrangement positioned the Y short-arm region at the distal end of the Y* chromosome and the pseudoautosomal region interstitially, just distal to the newly acquired centromere. In addition, the majority of the pseudoautosomal region was inverted. Recombination between the X and the Y* chromosomes generates two new sex chromosomes: (1) a large chromosome comprised of the X chromosome attached at its distal end to all of the Y* chromosome but missing the centromeric region (X(Y)*) and (2) a small chromosome containing the centromeric portion of the Y* chromosome attached to G-band-negative material from the X chromosome (Y*X). Mice that inherit the X(Y)* chromosome develop as sterile males, whereas mice that inherit the Y*X chromosome develop as fertile females. Recovery of equal numbers of recombinant and nonrecombinant offspring from X(Y)* males supports the hypothesis that recombination between the mammalian X and Y chromosomes is necessary for primary spermatocytes to successfully complete spermatogenesis and form functional sperm.