CHARACTERIZATION OF VAGAL INNERVATION TO THE RAT CELIAC, SUPRARENAL AND MESENTERIC GANGLIA
CHARACTERIZATION OF VAGAL INNERVATION TO THE RAT CELIAC, SUPRARENAL AND MESENTERIC GANGLIA
复制标题
DOI：
10.1016/0165-1838(93)90046-w
复制
发表时间：
1993-02-01
期刊：
影响因子：
--
通讯作者：
POWLEY, TL
中科院分区：
文献类型：
--
作者：
BERTHOUD, HR;POWLEY, TL
为了阐明有争议的迷走神经支配太阳丛神经节的问题，通过向运动背核（dmnX）注射荧光碳氰染料Dil，顺行标记迷走神经输出节前纤维。此外，氟金被用于标记神经节整体，提供了一种反染剂和紫外光引导下解剖各种神经节的可能性。用激光共聚焦显微镜对整个安装完好的神经节进行光学切片，发现在主要神经节（乳糜、肠系膜上和肾上）有相当数量的迪尔标记的迷走神经末梢样结构。此外，迷走神经传出末梢通常见于与腹腔动脉周围丛和肠系膜上动脉相关的小神经节，少数见于肠系膜间丛和肾丛的小神经节。通过使用先前选择性迷走神经分支迷走神经切断术的动物，只留下三个主要腹部分支中的一个（或一对）完整，我们得出结论，两个腹腔分支贡献了大部分迷走神经支配，两个胃分支和未配对的肝分支提供了一小部分，主要局限于腹腔神经节。对照实验中，将Dil注射到动物的dmnX中，这些动物的内脏事件先前被辣椒素破坏；(2)进入结节神经节，以便顺行标记迷走神经传入事件；(3)进入颈部迷走神经作为通道纤维摄取的控制，得出结论，鉴定的末梢样结构是迷走神经传出神经，而不是无意中标记的传入神经。我们认为这些迷走神经末梢必须被认为是异位副交感神经连接，或者是交感神经节传递的迷走神经门控机制的一部分。在功能上，太阳神经丛的副交感神经支配可能不仅对胃肠道靶点提供经典的迷走神经影响，而且还提供对肾上腺和其他传统上不被认为是迷走神经靶点的腹部器官的迷走神经控制。
In order to shed light on the controversial issue of vagal innervation of the solar plexus ganglia, vagal efferent preganglionic fibers were anterogradely labeled by injecting the fluorescent carbocyanine dye Dil into the dorsal motor nucleus (dmnX). Additionally, Fluorogold was used to label the ganglia in toto, providing a counterstain and the possibility of UV light-guided dissection of the various ganglia. Using optical sectioning of whole mounted intact ganglia by means of laser scanning confocal microscopy, a considerable number of Dil-labeled vagal terminal-like structures were found in the major ganglia (celiac, superior mesenteric and suprarenal). Additionally, vagal efferent terminals were regularly found in microganglia associated with the periarterial plexuses of the celiac and superior mesenteric arteries, and in a few cases in small ganglia of the intermesenteric and renal plexuses. By using animals with prior selective vagal branch vagotomies, leaving only one (or a pair) of the three major abdominal divisions intact, it was concluded that the two celiac branches contribute the bulk of this vagal innervation, with the two gastric and the unpaired hepatic branch providing a small contribution mostly limited to the celiac ganglia. From control experiments, which involved Dil injections (1) into the dmnX in animals whose visceral afferents had been previously destroyed by capsaicin; (2) into the nodose ganglia, in order to anterogradely label vagal afferents; and (3) into the cervical vagus nerve as a control for uptake by fibers of passage, it was concluded that the identified terminal-like structures were vagal efferents and not inadvertently labeled afferents. We suggest that these vagal terminals have to be regarded either as ectopic parasympathetic junctions, or as part of a vagal mechanism for gating of sympathetic ganglionic transmission. Functionally, the parasympathetic innervation of the solar plexus may provide not only the classic vagal influence on gastrointestinal targets, but also vagal control of the adrenal glands and possibly other abdominal organs that have not been traditionally regarded as vagal targets.