ADDITIONAL DNA IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS AND MOLECULAR-CLONING OF MEC-SPECIFIC DNA
ADDITIONAL DNA IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS AND MOLECULAR-CLONING OF MEC-SPECIFIC DNA
复制标题
DOI：
10.1128/jb.165.2.373-378.1986
复制
发表时间：
1986-02-01
影响因子：
3.2
通讯作者：
KAYSER, FH
中科院分区：
文献类型：
--
作者：
BECK, WD;BERGERBACHI, B;KAYSER, FH
通过对染色体DNA的一维和二维限制性内切酶分析，显示耐甲氧西林金黄色葡萄球菌中存在额外的DNA。将甲氧西林耐药菌株中存在但在甲氧西林敏感亲本菌株中不存在的3.5-乙酰氨基转移酶BglII片段克隆到大肠杆菌中新构建的质粒pWDB 1中。将该3.5-内切酶BglII片段与不同的甲氧西林敏感和耐甲氧西林的S.金黄色葡萄球菌临床分离株表明该片段代表甲氧西林抗性决定簇（MEC）的一部分。此外，该片段携带存在于一些大的葡萄球菌质粒以及青霉素酶质粒pI 524中的序列。
Additional DNA was shown to be present in methicillin-resistant Staphylococcus aureus by one- and two-dimensional restriction endonuclease analyses of the chromosomal DNA. A 3.5-kilobase BglII fragment, which was present in methicillin-resistant strains but not in the isogenic methicillin-sensitive parental strain, was cloned into newly constructed plasmid pWDB1 in Escherichia coli. Hybridization of this 3.5-kilobase BglII fragment with different methicillin-sensitive and methicillin-resistant S. aureus clinical isolates indicated that the fragment represents part of the methicillin resistance determinant (mec). In addition, the fragment carries a sequence that is present in some large staphylococcal plasmids, as well as in penicillinase plasmid pI524.