Mass spectrometric and high performance liquid chromatography profiling of the venom of the Brazilian vermivorous mollusk Conus regius:: feeding behavior and identification of one novel conotoxin
Mass spectrometric and high performance liquid chromatography profiling of the venom of the Brazilian vermivorous mollusk Conus regius:: feeding behavior and identification of one novel conotoxin
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DOI：
10.1016/j.toxicon.2004.09.018
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发表时间：
2005-01-01
期刊：
影响因子：
2.8
通讯作者：
Pimenta, DC
中科院分区：
文献类型：
--
作者：
Braga, MCV;Konno, K;Pimenta, DC
芋螺属的肉食性软体动物通过注射富含生物活性肽的混合物来麻痹猎物。王芋螺是该属的食虫性成员，栖息在巴西热带沃茨。锥螺毒液的种间、种内和个体变异以前已有报道。为了研究C.结果表明：1.王蝎毒液的种类、摄食行为及两者之间的相关性; 2.王蝎毒液的种类、摄食行为及两者之间的相关性。用高效液相色谱法对全毒和一个特异峰进行了监测。色谱图显示，其峰面积没有显着差异，表明毒液的组成，仅基于在存在或不存在的主峰，是稳定的，无论季节，性别和大小。因此，在一个主峰中洗脱的一种给定毒素的分析在群体中具有代表性。此外，本研究还鉴定了一种新的芋螺毒素rg 11 a，并通过质谱分析推测了其氨基酸序列。(C)2004爱思唯尔有限公司保留所有权利。
Carnivorous mollusks belonging to the genus Conus paralyze their prey by injecting a rich mixture of biologically active peptides. Conus regius is a vermivorous member of this genus that inhabits Brazilian tropical waters. Inter-, intraspecies and individual variations of cone snail venom have been previously reported. In order to investigate intra-specific differences in C. regius venom, its feeding behavior and the correlation between these two factors, animals were pooled according to gender, size and season of collection, and their venom composition was compared by high performance liquid chromatography (HPLC). Both the whole venom and one specific peak were monitored by HPLC. Chromatographic profiles revealed no significant differences in their peak areas, indicating that the venom composition, based solely in the presence or absence of the major peaks, is stable regardless of season, gender and size. Therefore, analysis of one given toxin, eluting in one of the major peaks, is representative among the population. Moreover, this work presents the identification of one novel conotoxin (rg11a), which amino acid sequence was deduced by mass spectrometry. (C) 2004 Elsevier Ltd. All rights reserved.