The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound.
The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound.
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DOI：
10.1177/0963689720965478
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发表时间：
2020-01
影响因子：
3.3
通讯作者：
Thakor AS
中科院分区：
文献类型：
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作者：
Razavi M;Rezaee M;Telichko A;Inan H;Dahl J;Demirci U;Thakor AS
我们研究了来自不同来源的间充质干细胞（MSCs）对脉冲聚焦超声（pFUS）的旁分泌功能。将人脂肪组织（AD）、骨髓（BM）和脐带（UC）来源的MSC暴露于两种强度的pFUS：0.45W/cm 2 ISATA（310 kPa PNP）和1.3W/cm 2 ISATA（540 kPa PNP）。pFUS后，使用血细胞计数器和共聚焦显微镜评估MSC的活力和增殖，并使用多重ELISA确定其分泌的细胞因子谱。我们的研究结果表明，pFUS可以刺激生产的免疫调节，抗炎和血管生成细胞因子的MSC，这是依赖于正在研究的MSC的来源和声强度。这些重要的发现为使用这种新的非侵入性和临床可转化技术调节MSC生物学的额外机制和验证研究奠定了基础。
We studied the paracrine function of mesenchymal stem cells (MSCs) derived from various sources in response to pulsed focused ultrasound (pFUS). Human adipose tissue (AD), bone marrow (BM), and umbilical cord (UC) derived MSCs were exposed to pFUS at two intensities: 0.45 W/cm2 ISATA (310 kPa PNP) and 1.3 W/cm2 ISATA (540 kPa PNP). Following pFUS, the viability and proliferation of MSCs were assessed using a hemocytometer and confocal microscopy, and their secreted cytokine profile determined using a multiplex ELISA. Our findings showed that pFUS can stimulate the production of immunomodulatory, anti-inflammatory, and angiogenic cytokines from MSCs which was dependent on both the source of MSC being studied and the acoustic intensity employed. These important findings set the foundation for additional mechanistic and validation studies using this novel noninvasive and clinically translatable technology for modulating MSC biology.