RNA editing regulates insect gamma-aminobutyric acid receptor function and insecticide sensitivity
RNA editing regulates insect gamma-aminobutyric acid receptor function and insecticide sensitivity
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DOI：
10.1097/wnr.0b013e32830216c7
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发表时间：
2008-06-11
期刊：
影响因子：
1.7
通讯作者：
Hamon, Alain
中科院分区：
文献类型：
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作者：
Es-Salah, Zeineb;Lapied, Bruno;Hamon, Alain
据报道，通过腺苷脱氨酶进行的A至I前mRNA编辑增强了神经系统中的蛋白质多样性。在果蝇中，对狄氏剂（RDL）γ-氨基丁酸（GABA）受体亚基的抗性显示了一个编辑位点（R122），该位点靠近假定的GABA结合位点。我们通过在非洲爪蟾卵母细胞中表达同源RDL受体来评估在该位点编辑的功能效应。用甘氨酸取代精氨酸122后，激动剂和非泼罗尼的效力在非泼罗尼敏感受体或突变的耐药受体（A301 G/T350 M）中向右偏移。这些数据首次揭示了RNA编辑对GABA受体功能的影响。
A-to-l pre-mRNA editing by adenosine deaminase enzymes has been reported to enhance protein diversity in the nervous system. In Drosophila, the resistance to dieldrin (RDL) gamma-aminobutyric acid (GABA) receptor subunit displays an editing site (R122) that is close to the putative GABA-binding site. We assessed the functional effects of editing at this site by expressing homomeric RDL receptors in Xenopus oocytes. After replacement of arginine 122 with a glycine, both agonist and fipronil potencies were shifted to the right in either fipronil-sensitive receptors or mutated resistant receptors (A301G/T350M). These data provide the first insight on the influence of RNA editing on GABA receptor function.