Modulation of scleral DNA synthesis in development of and recovery from induced axial myopia in the tree shrew
Modulation of scleral DNA synthesis in development of and recovery from induced axial myopia in the tree shrew
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DOI：
10.1006/exer.1998.0587
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发表时间：
1999-02-01
影响因子：
3.4
通讯作者：
McBrien, NA
中科院分区：
文献类型：
--
作者：
Gentle, A;McBrien, NA
视觉调节的巩膜细胞外基质重塑与树鼩（一种常用的屈光不正发展的哺乳动物模型）诱发的轴性近视的发展和恢复有关。用[H-3]胸腺嘧啶研究了巩膜细胞增殖在这一过程中的作用。将树鼩单眼剥夺模式视觉（使用半透明遮片）或视网膜图像光学散焦（使用负晶状体），为期5 d，另一组单眼剥夺5 d，然后双眼恢复3 d，对照组双眼开放以建立正常参数。在实验最后一天，腹腔注射[RH]胸苷嘧啶，然后进行光学和生物特征测量，并采集组织样本进行测定。在三氯乙酸沉淀后，用蛋白酶K消化法测定[RH]胸腺嘧啶与细胞DNA的结合。5天后，治疗后的两眼均出现了明显的近视[- 7.0 +/- 0.7屈光度(D)，组平均+/- S.E.M.；透镜离焦动物（- 6.2 +/- 0.9 D: P < 0.01）。恢复3天后，50%的屈光不正得到补偿，主要是通过缩短治疗眼的玻璃体腔。两组近视动物的巩膜中[H-3]胸腺嘧啶掺入水平均降低。- 34.3±9.9%；P < 0.05，透镜离焦- 32.8 +/- 4.5%；P < 0.005)。恢复动物巩膜[H-3]胸腺嘧啶掺入水平升高（+ 144.0 +/- 43.2%,P < 0.05），表明在近视发展过程中细胞增殖调节发生了变化。是视觉介导的，与眼大小变化的方向成反比。这表明在近视发展过程中，巩膜成纤维细胞群的改变参与了巩膜基质周转的调节。(C) 1999学术出版社。
Visually modulated scleral extracellular matrix remodelling is associated with the development of, and recovery ii om, induced axial myopia in the tree shrew, a commonly used mammalian model of refractive error development. The involvement of scleral cell proliferation in this process was investigated using [H-3] thymidine. Tree shrews were monocularly deprived of pattern vision, using translucent occluders, or the retinal image was optically defocused, using negative lenses, over a period of 5 days, A further group was monocularly deprived for 5 days, then allowed 3 days of binocular recovery, A control group of binocularly open animals was employed to establish normal parameters. On the final day of the experimental period, [RH] thymidine was administered by intraperitoneal injection, then optical and biometric measures were taken and tissue samples collected for assay. Incorporation of [RH] thymidine into cellular DNA was measured in proteinase K digests, following precipitation with trichloroacetic acid. After 5 days, significant amounts of myopia were present in the treated eyes of both form-deprived [- 7.0 +/- 0.7 Dioptres (D), group mean +/- S.E.M.; P < 0.01] and lens-defocused animals (- 6.2 +/- 0.9 D: P < 0.01). After 3 days of recovery, 50% of the refractive error had been compensated for, predominantly through shortening of the vitreous chamber in the treated eye. Reduced levels of [H-3] thymidine incorporation were observed in sclera From both groups of myopic animals (form-deprived. - 34.3 +/- 9.9%; P < 0.05 and lens-defocus, - 32.8 +/- 4.5%; P < 0.005). Increased levels of [H-3] thymidine incorporation were Found in the sclera of recovering animals (+ 144.0 +/- 43.2%; P < 0.05) The results show that changes in regulation of cell proliferation, during the development of myopia. are visually mediated and inversely related to the direction of change in ocular size. This implies that alterations in the scleral fibroblast population are involved in thr modulation of scleral matrix turnover during myopia development. (C) 1999 Academic Press.