Identification and localization of a novel ecdysone oxidase in the silkworm, <i>Bombyx mori</i>
Identification and localization of a novel ecdysone oxidase in the silkworm, <i>Bombyx mori</i>
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家蚕中一种新型蜕皮激素氧化酶的鉴定和定位，<i>Bombyx mori</i>
DOI：
10.11416/jibs.86.2_049
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发表时间：
2017
影响因子：
--
通讯作者：
Kohji Yamamoto and Sumiharu Nagaoka
中科院分区：
文献类型：
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作者：
Nagaoka;S.;Asagoshi;M.;Kato;K.;Takata;Y.;渡辺昌規，西山航生，塩野忠彦，楠信行;Kohji Yamamoto and Sumiharu Nagaoka
蜕皮激素氧化酶（EO）是一种催化昆虫蜕皮激素氧化的酶。该酶可能在蜕皮甾体转化为3-脱氢蜕皮甾体中起重要作用。在这项研究中，我们确定了一个新的EO，指定bmEO 2，从家蚕，家蚕。采用逆转录-聚合酶链反应（PCR）技术克隆了编码bmEO 2的cDNA。bmEO 2的氨基酸序列与已知的昆虫EOs和葡萄糖脱氢酶蛋白具有同源性。实时荧光定量PCR结果显示，bmEO 2基因在B幼虫睾丸中大量表达。森。
Ecdysone oxidase (EO) is an enzyme that catalyzes the oxidation of ecdysteroids, the molting steroid hormones in insects. This enzyme may play important roles in the conversion of ecdysteroids to 3-dehydroecdysteroids. In this study, we identified a novel EO, designated bmEO2, from the silkworm, Bombyx mori. A cDNA encoding bmEO2 was cloned by employing the reverse transcriptase-polymerase chain reaction (PCR) technique. The deduced amino acid sequence of bmEO2 showed conserved residues and sequence homology to other known insect EOs and glucose dehydrogenase proteins. Quantitative real-time PCR revealed that the bmEO2 transcript was abundantly present in larval testes of B. mori.