Sediment microbial community analysis: Establishing impacts of aquaculture on a tropical mangrove ecosystem
Sediment microbial community analysis: Establishing impacts of aquaculture on a tropical mangrove ecosystem
复制标题
DOI：
10.1016/j.aquaculture.2009.09.013
复制
发表时间：
2009-12-01
期刊：
影响因子：
4.5
通讯作者：
McKinnon, David A.
中科院分区：
文献类型：
--
作者：
Castine, Sarah A.;Bourne, David G.;McKinnon, David A.
通过比较网箱下和远离养殖场租赁区的梯度上表层沉积物（0-8.5 cm）中微生物群落，研究了热带鳍鱼网箱养殖场有机负荷的影响。无论是总细菌还是弧菌，分别通过落射荧光显微镜和TCBS平板计数，在笼部位比远离笼的对照部位更丰富。总细菌范围为1.1 x 10（7）个细胞g WW-1（笼部位）至1.9 x 10（6）个细胞g WW-1（远离笼部位），弧菌属丰度范围为1.6 x 10（5）CFU g WW-1（笼部位）至5.3 x 10（2）FU WW-1（远离笼部位）。细菌16 S rRNA基因克隆文库显示高度多样化的微生物群落，在门和子类隶属水平上，笼下和远离笼的位点具有相似的模式。然而，在笼下的位点观察到关键微生物组的16 S rRNA基因序列相对丰度的小变化。例如，与ε-变形菌门和弧菌属相关的16 S rRNA序列存在于笼位点，而在远离笼的位点不存在。此外，与远离笼子的站点相比，在笼子站点观察到与δ-变形菌门相关的序列的相对丰度更高。这项研究是第一个调查的影响，鳍鱼网箱养殖在热带海洋生态系统中的网箱下沉积物的微生物群落，并表明，微生物丰度的变化和沉积细菌群落的变化可能是潜在的有用的指标，在这些环境中的有机扰动。(c)2009爱思唯尔有限公司版权所有。
The impact of organic loading from a tropical finfish cage farm was investigated by comparing the microbial community in surface sediments (0-8.5 cm) at sites under fish cages and on a gradient away from the farm lease area. Both total bacteria and Vibrio sp., enumerated by epifluorescence microscopy and TCBS plate counts respectively, were more abundant at cage sites than at the control sites away from the cages. Total bacteria ranged from 1.1 x 10(7) cells g WW-1 at cage sites to 1.9 x 10(6) cells g WW-1 at sites away from the cages and Vibrio sp. abundance ranged from 1.6 x 10(5) CFU g WW-1 at cage sites to 5.3 x 10(2) FU WW-1 at sites away from the cages. Bacterial 16S rRNA gene clone libraries demonstrated highly diverse microbial communities with similar patterns at the phyla and sub-class affiliation level for sites under the cages and sites away from the cages. However, small shifts in relative abundance of 16S rRNA gene sequences of key microbial groups were observed for sites under the cages. For example, 16S rRNA sequences related to epsilon-Proteobacteria and Vibrio sp. were present at cage sites and absent at sites away from the cages. In addition, a higher relative abundance of sequences related to the delta-Proteobacteria were observed at cage sites compared to sites away from the cages. This study is the first to investigate impacts of finfish cage aquaculture on the microbial community of sediments under fish cages in tropical marine ecosystems and demonstrates that changes in microbial abundance and shifts in sedimentary bacterial communities may be potentially useful indicators of organic perturbations in these environments. (c) 2009 Elsevier B.V. All rights reserved.