γδTCR+ cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin
γδTCR+ cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin
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DOI：
10.1016/j.jri.2009.10.003
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发表时间：
2010-01-01
影响因子：
3.4
通讯作者：
Meeusen, Els N.
中科院分区：
文献类型：
--
作者：
Fox, Annette;Maddox, Jill F.;Meeusen, Els N.
伽玛-德尔塔T细胞是反刍动物妊娠子宫中的一种重要的颗粒细胞群，其数量和颗粒大小在妊娠期间都显著增加。用锚定RT-PCR方法检测妊娠绵羊子宫上皮细胞对TCR Delta基因的利用。获得的TCR Delta基因表现出明显的组合和连接多样性，测序的9个V-D-J重排中只有2个重排完全相同。此外，所使用的两个V Delta元素也在外周血中表达，这表明在绵羊上皮细胞中使用Gamma Delta TCR既不受限制，也不具有部位特异性，与人类相似，但与在小鼠中的发现相反。对纯化的颗粒化子宫伽马三角洲T细胞的蛋白质分析显示，存在大量的抗菌肽-颗粒溶素。本研究结果表明，绵羊子宫γ-TCR+上皮内淋巴细胞具有识别多种抗原的潜能，在妊娠和分娩期间可能具有保护子宫免受感染的作用。人类顶叶蜕膜中可能存在类似的伽马-德尔塔T细胞保护作用。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
gamma delta T cells are a prominent granulated cell population in the ruminant pregnant uterus and both their number and granule size increase dramatically during pregnancy. Anchor-RT-PCR was used to assess TCR delta gene usage by gamma delta T cells from the uterine epithelium of pregnant sheep. The TCR delta genes obtained exhibited distinct combinatorial and junctional diversity and only two out of nine V-D-J rearrangements sequenced were identical. Furthermore, two of the V delta elements used are also expressed in peripheral blood, indicating that gamma delta TCR use in sheep epithelia is neither restricted nor site-specific, similar to humans but in contrast to findings in mice. Protein analysis of purified, granulated uterine gamma delta T cells revealed the presence of large amounts of the antimicrobial peptide, granulysin. The results of the present study indicate that ovine uterine gamma delta TCR+ intraepithelial lymphocytes have the potential to recognise diverse antigens and may have a role in protecting the uterus from infection during pregnancy and parturition. A similar protective role for gamma delta T cells may exist in the human decidua parietalis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.