Fabrication and evaluation of nanoporous alumina membranes for osteoblast culture.
Fabrication and evaluation of nanoporous alumina membranes for osteoblast culture.
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DOI：
10.1002/jbm.a.30223
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发表时间：
2005-03
期刊：
影响因子：
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通讯作者：
E. E. L. Swan-E.;K. Popat;C. Grimes;T. Desai
中科院分区：
文献类型：
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作者：
E. E. L. Swan-E.;K. Popat;C. Grimes;T. Desai
An understanding of osteoblast response to surface topography is essential for successful bone tissue engineering applications. Alumina has been extensively used as a substrate for bone tissue constructs. However, current techniques do not allow precise surface topography and orientation of the material. In this research, a two-step anodization process was optimized for the fabrication of nanoporous alumina membranes with uniform pore dimension and distribution. The anodization voltage can be varied to create nanoporous alumina membranes with pore sizes ranging from 30 to 80 nm in diameter. The impact of the nanoscale pores on osteoblast response was studied by evaluating cell adhesion, morphology, and matrix production. Scanning electron microscopy and atomic force microscopy were used to characterize the nanoporous alumina membranes. Osteoblast adhesion and morphology were investigated using scanning electron microscopy images and matrix production was characterized using energy dispersive spectroscopy. This research combined the advantages of using alumina, a material with proven biocompatibility and current orthopedic clinical applications, and incorporated porous features on the nanoscale which have been reported to improve osteoblast response.
